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- W2041036779 abstract "Sigma factors are essential global regulators of transcription initiation in bacteria which confer promoter recognition specificity to the RNA polymerase core enzyme. They provide effective mechanisms for simultaneously regulating expression of large numbers of genes in response to challenging conditions, and their presence has been linked to bacterial virulence and pathogenicity. In this study, we constructed nine his-tagged sigma factor expressing and/or deletion mutant strains in the opportunistic pathogen Pseudomonas aeruginosa. To uncover the direct and indirect sigma factor regulons, we performed mRNA profiling, as well as chromatin immunoprecipitation coupled to high-throughput sequencing. We furthermore elucidated the de novo binding motif of each sigma factor, and validated the RNA- and ChIP-seq results by global motif searches in the proximity of transcriptional start sites (TSS). Our integrated approach revealed a highly modular network architecture which is composed of insulated functional sigma factor modules. Analysis of the interconnectivity of the various sigma factor networks uncovered a limited, but highly function-specific, crosstalk which orchestrates complex cellular processes. Our data indicate that the modular structure of sigma factor networks enables P. aeruginosa to function adequately in its environment and at the same time is exploited to build up higher-level functions by specific interconnections that are dominated by a participation of RpoN." @default.
- W2041036779 created "2016-06-24" @default.
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- W2041036779 date "2015-03-17" @default.
- W2041036779 modified "2023-10-17" @default.
- W2041036779 title "Elucidation of Sigma Factor-Associated Networks in Pseudomonas aeruginosa Reveals a Modular Architecture with Limited and Function-Specific Crosstalk" @default.
- W2041036779 cites W12119413 @default.
- W2041036779 cites W1509847467 @default.
- W2041036779 cites W1548309673 @default.
- W2041036779 cites W1550872310 @default.
- W2041036779 cites W1597786221 @default.
- W2041036779 cites W1627332943 @default.
- W2041036779 cites W1644749979 @default.
- W2041036779 cites W1674719483 @default.
- W2041036779 cites W1894518824 @default.
- W2041036779 cites W1924447801 @default.
- W2041036779 cites W1931330974 @default.
- W2041036779 cites W1947636021 @default.
- W2041036779 cites W1949208903 @default.
- W2041036779 cites W1956643415 @default.
- W2041036779 cites W1967641124 @default.
- W2041036779 cites W1970790687 @default.
- W2041036779 cites W1971584852 @default.
- W2041036779 cites W1975587832 @default.
- W2041036779 cites W1978334623 @default.
- W2041036779 cites W1985687704 @default.
- W2041036779 cites W1987091690 @default.
- W2041036779 cites W1989380134 @default.
- W2041036779 cites W1992204008 @default.
- W2041036779 cites W1999251928 @default.
- W2041036779 cites W2001164252 @default.
- W2041036779 cites W2001394772 @default.
- W2041036779 cites W2002975202 @default.
- W2041036779 cites W2014630952 @default.
- W2041036779 cites W2014751502 @default.
- W2041036779 cites W2014937949 @default.
- W2041036779 cites W2017266538 @default.
- W2041036779 cites W2018333631 @default.
- W2041036779 cites W2020057555 @default.
- W2041036779 cites W2026379227 @default.
- W2041036779 cites W2034279867 @default.
- W2041036779 cites W2036335453 @default.
- W2041036779 cites W2038897801 @default.
- W2041036779 cites W2040960801 @default.
- W2041036779 cites W2047023007 @default.
- W2041036779 cites W2055109879 @default.
- W2041036779 cites W2057961335 @default.
- W2041036779 cites W2058358568 @default.
- W2041036779 cites W2060913542 @default.
- W2041036779 cites W2062896692 @default.
- W2041036779 cites W2075716499 @default.
- W2041036779 cites W2076070614 @default.
- W2041036779 cites W2077460182 @default.
- W2041036779 cites W2078512217 @default.
- W2041036779 cites W2078569492 @default.
- W2041036779 cites W2086358238 @default.
- W2041036779 cites W2091774406 @default.
- W2041036779 cites W2095570626 @default.
- W2041036779 cites W2098669559 @default.
- W2041036779 cites W2104817697 @default.
- W2041036779 cites W2105097251 @default.
- W2041036779 cites W2105868980 @default.
- W2041036779 cites W2108553019 @default.
- W2041036779 cites W2109038233 @default.
- W2041036779 cites W2110837877 @default.
- W2041036779 cites W2118342782 @default.
- W2041036779 cites W2124786260 @default.
- W2041036779 cites W2124985265 @default.
- W2041036779 cites W2130764574 @default.
- W2041036779 cites W2131417813 @default.
- W2041036779 cites W2133212095 @default.
- W2041036779 cites W2140498399 @default.
- W2041036779 cites W2140952049 @default.
- W2041036779 cites W2143372407 @default.
- W2041036779 cites W2144722775 @default.
- W2041036779 cites W2144748915 @default.
- W2041036779 cites W2147171429 @default.
- W2041036779 cites W2147498477 @default.
- W2041036779 cites W2149353640 @default.
- W2041036779 cites W2150093407 @default.
- W2041036779 cites W2152239989 @default.
- W2041036779 cites W2153582281 @default.
- W2041036779 cites W2154454968 @default.
- W2041036779 cites W2155954664 @default.
- W2041036779 cites W2156353316 @default.
- W2041036779 cites W2157009395 @default.
- W2041036779 cites W2158804744 @default.
- W2041036779 cites W2162376398 @default.
- W2041036779 cites W2162851174 @default.