Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041056598> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2041056598 abstract "Total RNA was extracted from mature and juvenile Fasciola hepatica by homogenizing in 5.0 M guanidine thiocyanate and centrifugation through a 5.7 M CsCl cushion. Yields of 2 mg/g and 1 mg/g wet weight starting material were obtained, respectively. Messenger RNA was separated from the bulk extracted RNA by binding to oligo(dT)-cellulose. About 25% of the extracted RNA bound in both adult and juvenile cases. This material was subsequently tested in a rabbit reticulocyte cell-free translation system. Up to a 12-fold stimulation of incorporation of [35S]methionine into trichloroacetic acid-precipitable material was observed over that where no message was added. When the in vitro translation products were analysed by autoradiography of SDS-polyacrylamide gradient gels, polypeptides ranging in apparent molecular weight from about 10,000 to 100,000 were observed. Several minor differences in the electrophoretic patterns obtained from juvenile and adult mRNA were observed." @default.
- W2041056598 created "2016-06-24" @default.
- W2041056598 creator A5031376626 @default.
- W2041056598 creator A5072034032 @default.
- W2041056598 date "1981-12-01" @default.
- W2041056598 modified "2023-09-23" @default.
- W2041056598 title "Preparation and in vitro translation of mRNA from Fasciola hepatica" @default.
- W2041056598 cites W1605968041 @default.
- W2041056598 cites W167787940 @default.
- W2041056598 cites W1967358192 @default.
- W2041056598 cites W1970403529 @default.
- W2041056598 cites W2007694238 @default.
- W2041056598 cites W2017729692 @default.
- W2041056598 cites W2032064293 @default.
- W2041056598 cites W2037287326 @default.
- W2041056598 cites W2043555649 @default.
- W2041056598 cites W2060333964 @default.
- W2041056598 cites W2066160869 @default.
- W2041056598 cites W2069239718 @default.
- W2041056598 cites W2076892293 @default.
- W2041056598 cites W2077481808 @default.
- W2041056598 cites W2081708147 @default.
- W2041056598 cites W2100837269 @default.
- W2041056598 cites W2171981785 @default.
- W2041056598 cites W2317697683 @default.
- W2041056598 cites W2320783457 @default.
- W2041056598 cites W2420158158 @default.
- W2041056598 cites W73502799 @default.
- W2041056598 doi "https://doi.org/10.1016/0166-6851(81)90065-7" @default.
- W2041056598 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6121288" @default.
- W2041056598 hasPublicationYear "1981" @default.
- W2041056598 type Work @default.
- W2041056598 sameAs 2041056598 @default.
- W2041056598 citedByCount "12" @default.
- W2041056598 crossrefType "journal-article" @default.
- W2041056598 hasAuthorship W2041056598A5031376626 @default.
- W2041056598 hasAuthorship W2041056598A5072034032 @default.
- W2041056598 hasConcept C104317684 @default.
- W2041056598 hasConcept C105580179 @default.
- W2041056598 hasConcept C149364088 @default.
- W2041056598 hasConcept C153911025 @default.
- W2041056598 hasConcept C165901193 @default.
- W2041056598 hasConcept C181199279 @default.
- W2041056598 hasConcept C202751555 @default.
- W2041056598 hasConcept C203014093 @default.
- W2041056598 hasConcept C2777856171 @default.
- W2041056598 hasConcept C2778403015 @default.
- W2041056598 hasConcept C2778476535 @default.
- W2041056598 hasConcept C2780912031 @default.
- W2041056598 hasConcept C3675279 @default.
- W2041056598 hasConcept C515207424 @default.
- W2041056598 hasConcept C55493867 @default.
- W2041056598 hasConcept C58121356 @default.
- W2041056598 hasConcept C65666806 @default.
- W2041056598 hasConcept C67705224 @default.
- W2041056598 hasConcept C7623868 @default.
- W2041056598 hasConcept C86803240 @default.
- W2041056598 hasConcept C88478588 @default.
- W2041056598 hasConceptScore W2041056598C104317684 @default.
- W2041056598 hasConceptScore W2041056598C105580179 @default.
- W2041056598 hasConceptScore W2041056598C149364088 @default.
- W2041056598 hasConceptScore W2041056598C153911025 @default.
- W2041056598 hasConceptScore W2041056598C165901193 @default.
- W2041056598 hasConceptScore W2041056598C181199279 @default.
- W2041056598 hasConceptScore W2041056598C202751555 @default.
- W2041056598 hasConceptScore W2041056598C203014093 @default.
- W2041056598 hasConceptScore W2041056598C2777856171 @default.
- W2041056598 hasConceptScore W2041056598C2778403015 @default.
- W2041056598 hasConceptScore W2041056598C2778476535 @default.
- W2041056598 hasConceptScore W2041056598C2780912031 @default.
- W2041056598 hasConceptScore W2041056598C3675279 @default.
- W2041056598 hasConceptScore W2041056598C515207424 @default.
- W2041056598 hasConceptScore W2041056598C55493867 @default.
- W2041056598 hasConceptScore W2041056598C58121356 @default.
- W2041056598 hasConceptScore W2041056598C65666806 @default.
- W2041056598 hasConceptScore W2041056598C67705224 @default.
- W2041056598 hasConceptScore W2041056598C7623868 @default.
- W2041056598 hasConceptScore W2041056598C86803240 @default.
- W2041056598 hasConceptScore W2041056598C88478588 @default.
- W2041056598 hasLocation W20410565981 @default.
- W2041056598 hasLocation W20410565982 @default.
- W2041056598 hasOpenAccess W2041056598 @default.
- W2041056598 hasPrimaryLocation W20410565981 @default.
- W2041056598 hasRelatedWork W151447238 @default.
- W2041056598 hasRelatedWork W1976699551 @default.
- W2041056598 hasRelatedWork W1994628553 @default.
- W2041056598 hasRelatedWork W2023825242 @default.
- W2041056598 hasRelatedWork W2032764549 @default.
- W2041056598 hasRelatedWork W2032977728 @default.
- W2041056598 hasRelatedWork W2041056598 @default.
- W2041056598 hasRelatedWork W2045431978 @default.
- W2041056598 hasRelatedWork W2046489735 @default.
- W2041056598 hasRelatedWork W2094444433 @default.
- W2041056598 isParatext "false" @default.
- W2041056598 isRetracted "false" @default.
- W2041056598 magId "2041056598" @default.
- W2041056598 workType "article" @default.