FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041103640> ?p ?o ?g. }

• W2041103640 endingPage "E604" @default.
• W2041103640 startingPage "E594" @default.
• W2041103640 abstract "The goal of this study was to investigate the entrapment of 3 different model proteins (tetanus toxoid, lysozyme, and insulin) into poly(D,L-lactic acid) and poly(D,L-lactic-co-glycolic acid) nanoparticles and to address process-related stability issues. For that purpose, a modified nanoprecipitation method as well as 2 emulsion-based encapsulation techniques (ie, a solid-in oil-in water (s/o/w) and a double emulsion (w1/o/w2) method) were used. The main modification of nanoprecipitation involved the use of a wide range of miscible organic solvents such as dimethylsulfoxide and ethanol instead of the common acetone and water. The results obtained showed that tetanus toxoid and lysozyme were efficiently incorporated by the double emulsion procedure when ethyl acetate was used as solvent (>80% entrapment efficiency), whereas it was necessary to use methylene chloride to achieve high insulin entrapment efficiencies. The use of the s/o/w method or the formation of a more hydrophobic protein-surfactant ion pair did not improve protein loading. The nanoprecipitation method led to a homogenous population of small nanoparticles (with size ranging from ≈130 to 560 nm) and in some cases also improved experimental drug loadings, especially for lysozyme (entrapment efficiency >90%). With respect to drug content determination, a simple and quick matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method provided results very close to those obtained by reverse phase-high-performance liquid chromatography. With respect to protein stability, the duration and intensity of sonication were not a concern for tetanus toxoid, which retained more than 95% of its antigenicity after treatment for 1 minute. Only a high methylene chloride:water ratio was shown to slightly decrease toxoid antigenicity. Finally, no more than 3.3% of A21 desamido insulin and only traces of covalent insulin dimer were detected in nanoparticles. In conclusion, both the double emulsion and nanoprecipitation methods allowed efficient protein encapsulation. MALDI-TOF MS allowed accurate drug content determination. The manufacturing processes evaluated did not damage the primary structure of insulin." @default.
• W2041103640 created "2016-06-24" @default.
• W2041103640 creator A5025087607 @default.
• W2041103640 creator A5048496252 @default.
• W2041103640 creator A5057757155 @default.
• W2041103640 date "2005-12-01" @default.
• W2041103640 modified "2023-10-14" @default.
• W2041103640 title "Nanoprecipitation versus emulsion-based techniques for the encapsulation of proteins into biodegradable nanoparticles and process-related stability issues" @default.
• W2041103640 cites W111664466 @default.
• W2041103640 cites W1499803005 @default.
• W2041103640 cites W1558228970 @default.
• W2041103640 cites W1580991720 @default.
• W2041103640 cites W1582227496 @default.
• W2041103640 cites W1810520060 @default.
• W2041103640 cites W1953131195 @default.
• W2041103640 cites W1968533576 @default.
• W2041103640 cites W1983052470 @default.
• W2041103640 cites W1988172459 @default.
• W2041103640 cites W1990409558 @default.
• W2041103640 cites W1992353776 @default.
• W2041103640 cites W1997899946 @default.
• W2041103640 cites W2003530563 @default.
• W2041103640 cites W2010367363 @default.
• W2041103640 cites W2022580407 @default.
• W2041103640 cites W2025985753 @default.
• W2041103640 cites W2032247822 @default.
• W2041103640 cites W2038375609 @default.
• W2041103640 cites W2039419728 @default.
• W2041103640 cites W2046013354 @default.
• W2041103640 cites W2047369124 @default.
• W2041103640 cites W2050700167 @default.
• W2041103640 cites W2053647015 @default.
• W2041103640 cites W2065800254 @default.
• W2041103640 cites W2071543666 @default.
• W2041103640 cites W2074209949 @default.
• W2041103640 cites W2093962452 @default.
• W2041103640 cites W2095438503 @default.
• W2041103640 cites W2100633797 @default.
• W2041103640 cites W2125384222 @default.
• W2041103640 cites W2139232882 @default.
• W2041103640 cites W2144907950 @default.
• W2041103640 cites W2150855779 @default.
• W2041103640 cites W2152230026 @default.
• W2041103640 cites W2154043786 @default.
• W2041103640 cites W2161127726 @default.
• W2041103640 cites W2161195188 @default.
• W2041103640 cites W2164757273 @default.
• W2041103640 cites W248316178 @default.
• W2041103640 cites W3199261357 @default.
• W2041103640 cites W60307892 @default.
• W2041103640 doi "https://doi.org/10.1208/pt060474" @default.
• W2041103640 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2750607" @default.
• W2041103640 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16408861" @default.
• W2041103640 hasPublicationYear "2005" @default.
• W2041103640 type Work @default.
• W2041103640 sameAs 2041103640 @default.
• W2041103640 citedByCount "125" @default.
• W2041103640 countsByYear W20411036402012 @default.
• W2041103640 countsByYear W20411036402013 @default.
• W2041103640 countsByYear W20411036402014 @default.
• W2041103640 countsByYear W20411036402015 @default.
• W2041103640 countsByYear W20411036402016 @default.
• W2041103640 countsByYear W20411036402017 @default.
• W2041103640 countsByYear W20411036402018 @default.
• W2041103640 countsByYear W20411036402019 @default.
• W2041103640 countsByYear W20411036402020 @default.
• W2041103640 countsByYear W20411036402021 @default.
• W2041103640 countsByYear W20411036402022 @default.
• W2041103640 countsByYear W20411036402023 @default.
• W2041103640 crossrefType "journal-article" @default.
• W2041103640 hasAuthorship W2041103640A5025087607 @default.
• W2041103640 hasAuthorship W2041103640A5048496252 @default.
• W2041103640 hasAuthorship W2041103640A5057757155 @default.
• W2041103640 hasBestOaLocation W20411036402 @default.
• W2041103640 hasConcept C104628117 @default.
• W2041103640 hasConcept C127413603 @default.
• W2041103640 hasConcept C155672457 @default.
• W2041103640 hasConcept C178790620 @default.
• W2041103640 hasConcept C185592680 @default.
• W2041103640 hasConcept C2775920511 @default.
• W2041103640 hasConcept C2776966407 @default.
• W2041103640 hasConcept C2778123984 @default.
• W2041103640 hasConcept C2780165375 @default.
• W2041103640 hasConcept C2780471494 @default.
• W2041103640 hasConcept C2780472526 @default.
• W2041103640 hasConcept C2781213060 @default.
• W2041103640 hasConcept C42360764 @default.
• W2041103640 hasConcept C43617362 @default.
• W2041103640 hasConcept C523546767 @default.
• W2041103640 hasConcept C54355233 @default.
• W2041103640 hasConcept C55493867 @default.
• W2041103640 hasConcept C86803240 @default.
• W2041103640 hasConceptScore W2041103640C104628117 @default.
• W2041103640 hasConceptScore W2041103640C127413603 @default.
• W2041103640 hasConceptScore W2041103640C155672457 @default.
• W2041103640 hasConceptScore W2041103640C178790620 @default.
• W2041103640 hasConceptScore W2041103640C185592680 @default.
• W2041103640 hasConceptScore W2041103640C2775920511 @default.

• next