Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041129197> ?p ?o ?g. }
- W2041129197 endingPage "472" @default.
- W2041129197 startingPage "461" @default.
- W2041129197 abstract "Glutamine synthetase (EC 6.3.1.2) has been purified from Bacillus stearothermophilus. The molecular weight of the enzyme was found to be 630 000, and that of a component obtained on treating the enzyme with 4 M urea or 1% sodium dodecyl-sulfate plus 10 mM 2-mercaptoethanol 540 000 or 500 000, respectively, suggesting that the enzyme consists of 12 subunits. The enzyme requires divalent cations for activity, Mg2+ being the most effective activator. The pH and temperature optima in the presence of Mg2+ or Mn2+ are 7.3 or 6.5 and 70 or 75 °C, respectively. The enzyme is inactivated on exposure to 70 °C, but the inactivation is partially protected by Mg2+ (Mn2+ or glutamate) and completely by Mg2+ (Mn2+), NH4Cl and glutamate. Thermodynamic quantities for the enzyme reaction show a conformational transition at 58 °C. Glycine, alanine, serine, tryptophan, histidine, AMP and CTP inhibit the enzyme in the presence of Mg2+ or Mn2+. The inhibition of the Mg2+- or Mn2+-activated enzyme by these compounds seems to be cumulative, except for the combined effects of amino acids on the Mn2+-activated enzyme. Circular dichroism analyses of the enzyme show an α-helix, ß-structure and unfolded conformation. Addition of Mg2+ or Mn2+ results in an increase of the α-helix content accompanied by a decrease of the unfolded conformation content." @default.
- W2041129197 created "2016-06-24" @default.
- W2041129197 creator A5007640658 @default.
- W2041129197 creator A5017201838 @default.
- W2041129197 creator A5041808200 @default.
- W2041129197 creator A5050016825 @default.
- W2041129197 creator A5052954532 @default.
- W2041129197 date "1974-06-01" @default.
- W2041129197 modified "2023-10-17" @default.
- W2041129197 title "Purification and properties of glutamine synthetase from Bacillus stearothermophilus" @default.
- W2041129197 cites W1506233309 @default.
- W2041129197 cites W1506843698 @default.
- W2041129197 cites W1524061278 @default.
- W2041129197 cites W1573089231 @default.
- W2041129197 cites W1583226380 @default.
- W2041129197 cites W1585033241 @default.
- W2041129197 cites W1599681228 @default.
- W2041129197 cites W1625660792 @default.
- W2041129197 cites W1674926809 @default.
- W2041129197 cites W1767907712 @default.
- W2041129197 cites W1775749144 @default.
- W2041129197 cites W1938718775 @default.
- W2041129197 cites W1972013347 @default.
- W2041129197 cites W1973956782 @default.
- W2041129197 cites W2008507431 @default.
- W2041129197 cites W2016066638 @default.
- W2041129197 cites W2026108914 @default.
- W2041129197 cites W2034335419 @default.
- W2041129197 cites W2038560836 @default.
- W2041129197 cites W2041572942 @default.
- W2041129197 cites W2054373686 @default.
- W2041129197 cites W2061532551 @default.
- W2041129197 cites W2062228103 @default.
- W2041129197 cites W2067462759 @default.
- W2041129197 cites W2071077805 @default.
- W2041129197 cites W2087429313 @default.
- W2041129197 cites W2094572572 @default.
- W2041129197 cites W2111301002 @default.
- W2041129197 cites W2132068224 @default.
- W2041129197 cites W2305449604 @default.
- W2041129197 cites W95056594 @default.
- W2041129197 doi "https://doi.org/10.1016/0005-2744(74)90522-1" @default.
- W2041129197 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4152611" @default.
- W2041129197 hasPublicationYear "1974" @default.
- W2041129197 type Work @default.
- W2041129197 sameAs 2041129197 @default.
- W2041129197 citedByCount "24" @default.
- W2041129197 crossrefType "journal-article" @default.
- W2041129197 hasAuthorship W2041129197A5007640658 @default.
- W2041129197 hasAuthorship W2041129197A5017201838 @default.
- W2041129197 hasAuthorship W2041129197A5041808200 @default.
- W2041129197 hasAuthorship W2041129197A5050016825 @default.
- W2041129197 hasAuthorship W2041129197A5052954532 @default.
- W2041129197 hasConcept C133571119 @default.
- W2041129197 hasConcept C178790620 @default.
- W2041129197 hasConcept C181199279 @default.
- W2041129197 hasConcept C185592680 @default.
- W2041129197 hasConcept C2776706248 @default.
- W2041129197 hasConcept C2778460671 @default.
- W2041129197 hasConcept C2778913249 @default.
- W2041129197 hasConcept C2779289688 @default.
- W2041129197 hasConcept C2779349466 @default.
- W2041129197 hasConcept C28212737 @default.
- W2041129197 hasConcept C515207424 @default.
- W2041129197 hasConcept C55493867 @default.
- W2041129197 hasConcept C71240020 @default.
- W2041129197 hasConcept C74884574 @default.
- W2041129197 hasConceptScore W2041129197C133571119 @default.
- W2041129197 hasConceptScore W2041129197C178790620 @default.
- W2041129197 hasConceptScore W2041129197C181199279 @default.
- W2041129197 hasConceptScore W2041129197C185592680 @default.
- W2041129197 hasConceptScore W2041129197C2776706248 @default.
- W2041129197 hasConceptScore W2041129197C2778460671 @default.
- W2041129197 hasConceptScore W2041129197C2778913249 @default.
- W2041129197 hasConceptScore W2041129197C2779289688 @default.
- W2041129197 hasConceptScore W2041129197C2779349466 @default.
- W2041129197 hasConceptScore W2041129197C28212737 @default.
- W2041129197 hasConceptScore W2041129197C515207424 @default.
- W2041129197 hasConceptScore W2041129197C55493867 @default.
- W2041129197 hasConceptScore W2041129197C71240020 @default.
- W2041129197 hasConceptScore W2041129197C74884574 @default.
- W2041129197 hasIssue "2" @default.
- W2041129197 hasLocation W20411291971 @default.
- W2041129197 hasLocation W20411291972 @default.
- W2041129197 hasOpenAccess W2041129197 @default.
- W2041129197 hasPrimaryLocation W20411291971 @default.
- W2041129197 hasRelatedWork W1877940841 @default.
- W2041129197 hasRelatedWork W1962880047 @default.
- W2041129197 hasRelatedWork W1988259208 @default.
- W2041129197 hasRelatedWork W1991256377 @default.
- W2041129197 hasRelatedWork W2065172938 @default.
- W2041129197 hasRelatedWork W2119306119 @default.
- W2041129197 hasRelatedWork W2145949410 @default.
- W2041129197 hasRelatedWork W2150080332 @default.
- W2041129197 hasRelatedWork W2965295969 @default.
- W2041129197 hasRelatedWork W4205670493 @default.
- W2041129197 hasVolume "350" @default.
- W2041129197 isParatext "false" @default.