Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041182023> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2041182023 endingPage "99" @default.
- W2041182023 startingPage "91" @default.
- W2041182023 abstract "A molecular probe pSPAv6.2(+), with concatameric insert representing 6.2-times repeated copy of potato spindle tuber viroid (PSTV) RNA, was labelled with digoxigenin and used to detect PSTV by dot-blot hybridization assay. The probe was highly sensitive and specific, detecting as little as 2.5 pg of PSTV RNA. Both severe and mild PSTV strains were detectable in 64-512-times diluted crude extracts from infected tomato leaves, and potato leaves, sprouts, and seeds. For extraction of plant tissue three buffers were compared to determine the lowest non-specific background and the highest sensitivity. The results showed that the digoxigenin-labelled probe is as sensitive as the 32P-labelled probe and can replace radioactive techniques in PSTV detection. With such high sensitivity, the probe is also potentially useful for detecting the viroid in composite samples of mass-indexing programs." @default.
- W2041182023 created "2016-06-24" @default.
- W2041182023 creator A5041307831 @default.
- W2041182023 creator A5066794546 @default.
- W2041182023 date "1992-09-01" @default.
- W2041182023 modified "2023-09-27" @default.
- W2041182023 title "Highly sensitive digoxigenin-labelled DNA probe for the detection of potato spindle tuber viroid" @default.
- W2041182023 cites W1941537992 @default.
- W2041182023 cites W1964033350 @default.
- W2041182023 cites W1976787463 @default.
- W2041182023 cites W1987821888 @default.
- W2041182023 cites W1998751317 @default.
- W2041182023 cites W2014669590 @default.
- W2041182023 cites W2026929564 @default.
- W2041182023 cites W2039020873 @default.
- W2041182023 cites W2076377242 @default.
- W2041182023 cites W2078799229 @default.
- W2041182023 cites W2080517659 @default.
- W2041182023 cites W2086071394 @default.
- W2041182023 cites W2088215956 @default.
- W2041182023 cites W2092159592 @default.
- W2041182023 cites W2095421467 @default.
- W2041182023 cites W2173015395 @default.
- W2041182023 doi "https://doi.org/10.1016/0166-0934(92)90128-z" @default.
- W2041182023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1430068" @default.
- W2041182023 hasPublicationYear "1992" @default.
- W2041182023 type Work @default.
- W2041182023 sameAs 2041182023 @default.
- W2041182023 citedByCount "21" @default.
- W2041182023 countsByYear W20411820232013 @default.
- W2041182023 countsByYear W20411820232014 @default.
- W2041182023 countsByYear W20411820232015 @default.
- W2041182023 countsByYear W20411820232021 @default.
- W2041182023 crossrefType "journal-article" @default.
- W2041182023 hasAuthorship W2041182023A5041307831 @default.
- W2041182023 hasAuthorship W2041182023A5066794546 @default.
- W2041182023 hasConcept C104317684 @default.
- W2041182023 hasConcept C105580179 @default.
- W2041182023 hasConcept C112190416 @default.
- W2041182023 hasConcept C130197536 @default.
- W2041182023 hasConcept C153911025 @default.
- W2041182023 hasConcept C2776552150 @default.
- W2041182023 hasConcept C2780757758 @default.
- W2041182023 hasConcept C2780781446 @default.
- W2041182023 hasConcept C552990157 @default.
- W2041182023 hasConcept C55493867 @default.
- W2041182023 hasConcept C67705224 @default.
- W2041182023 hasConcept C81439078 @default.
- W2041182023 hasConcept C86803240 @default.
- W2041182023 hasConceptScore W2041182023C104317684 @default.
- W2041182023 hasConceptScore W2041182023C105580179 @default.
- W2041182023 hasConceptScore W2041182023C112190416 @default.
- W2041182023 hasConceptScore W2041182023C130197536 @default.
- W2041182023 hasConceptScore W2041182023C153911025 @default.
- W2041182023 hasConceptScore W2041182023C2776552150 @default.
- W2041182023 hasConceptScore W2041182023C2780757758 @default.
- W2041182023 hasConceptScore W2041182023C2780781446 @default.
- W2041182023 hasConceptScore W2041182023C552990157 @default.
- W2041182023 hasConceptScore W2041182023C55493867 @default.
- W2041182023 hasConceptScore W2041182023C67705224 @default.
- W2041182023 hasConceptScore W2041182023C81439078 @default.
- W2041182023 hasConceptScore W2041182023C86803240 @default.
- W2041182023 hasIssue "1-2" @default.
- W2041182023 hasLocation W20411820231 @default.
- W2041182023 hasLocation W20411820232 @default.
- W2041182023 hasOpenAccess W2041182023 @default.
- W2041182023 hasPrimaryLocation W20411820231 @default.
- W2041182023 hasRelatedWork W1988067284 @default.
- W2041182023 hasRelatedWork W2002473487 @default.
- W2041182023 hasRelatedWork W2008210873 @default.
- W2041182023 hasRelatedWork W2023859593 @default.
- W2041182023 hasRelatedWork W2034036767 @default.
- W2041182023 hasRelatedWork W2041182023 @default.
- W2041182023 hasRelatedWork W2077636498 @default.
- W2041182023 hasRelatedWork W2078359567 @default.
- W2041182023 hasRelatedWork W52248404 @default.
- W2041182023 hasRelatedWork W657236858 @default.
- W2041182023 hasVolume "39" @default.
- W2041182023 isParatext "false" @default.
- W2041182023 isRetracted "false" @default.
- W2041182023 magId "2041182023" @default.
- W2041182023 workType "article" @default.