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- W2041185540 abstract "The wood keratine intermediate filament proteins were isolated as their S -carboxymethyl derivatives ( S -carboxymethylkerateine A, SCMKA) and purified by gel filtration to remove residual non-helical protein of low molecular weight. The α-helix content of purified SCMKA was ∼ 62% in aggreement with that predicted for the α-helical coiled-coil segments from the amino acid sequences of the subunits. In aqueous buffer at pH 11 or in n-propanol (20% v/v) at pH 9.2 very large aggregates are dissociated and SCMKA exists largely as a mixture of the dimer (two-chain coiled-coil of M r ∼ 10300) and the tetramer. The protein species are not rapidly reversible equilibrium as judged from gel filtration and sedimentation equilibrium. It is probable that species with a range of association constants are present. The equilibrium is shifted towards the dimer with change of pH from 9.2 to 11 or by the addition of 20% (v/v) n-propanol. The tetrameric proteolytic digestion product which is derived from the 1B segment of the α-helical rod section of the keratin molecule dissociates in a similar way to intact SCMKA with increase of pH and in the presence of n-propanol. This indicates the importance of this region of the rod domain in the initial stages of the assembly of the filament. Electrostatic and hydrophobic interactions are implicated in the association of the two-chain coiled-coil to the tetramer both in intact SCMKA and the 1B segment tetramer. The results are discussed in relation to the intact dimeric and tetrameric complexes obtained from other intermediate filament types." @default.
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- W2041185540 date "1989-10-01" @default.
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- W2041185540 title "Aggregation of wool keratin intermediate filament proteins" @default.
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- W2041185540 doi "https://doi.org/10.1016/0141-8130(89)90020-2" @default.
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