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- W2041193313 abstract "A novel procedure for the quantitative isolation and purification of acyl-coenzyme A esters is presented. The procedure involves two steps: (1) tissue extraction using acetonitrile/2-propanol (3+1, v+v) followed by 0.1 M potassium phosphate, pH 6.7, and (2) purification using 2-(2-pyridyl)ethyl-functionalized silica gel. Recoveries determined by adding radiolabeled acetyl-, malonyl-, octanoyl-, oleoyl-, palmitoyl-, or arachidonyl-coenzyme A to powdered rat liver varied 93–104% for tissue extraction and 83–90% for solid-phase extraction. The procedure described allows for isolation and purification, with high recoveries, of acyl-coenzyme A esters differing widely in chain length and saturation." @default.
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- W2041193313 date "2008-05-01" @default.
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- W2041193313 title "Novel isolation procedure for short-, medium-, and long-chain acyl-coenzyme A esters from tissue" @default.
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- W2041193313 doi "https://doi.org/10.1016/j.ab.2008.02.022" @default.
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