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- W2041205933 abstract "Perfused bovine adrenal glands were labelled with 3H-adenosine or 32P-phosphate. The time course of the labelling and the nature of the labelled products in the various subcellular fractions were investigated. The catecholamine storing vesicles (chromaffin granules) took up labelled ATP, but significant amounts of 3H-ATP did not appear until 30 min after the injection of the isotope. Four hours after the injection of the isotope the predominantly labelled nucleotide in adrenal medulla was 3H-ATP which was specifically confined to chromaffin granules. There was no evidence that chromaffin granules accumulated significant amounts of 3H-ADP and 3H-AMP. Similar results were obtained with 32P-phosphate as a precursor. Inhibitors of mitochondrial oxidative phosphorylation (cyanide, dinitrophenol) almost completely prevented the uptake of 3H-ATP in chromaffin granules, whereas iodoacetic acid was less effective. It is concluded that in intact cells chromaffin granules obtain newly synthesized ATP mainly from a mitochondrial pool. The mechanism of ATP uptake into chromaffin granules now warrants a detailed study on isolated granules incubated in vitro." @default.
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- W2041205933 date "1976-02-01" @default.
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- W2041205933 title "Synthesis of nucleotides in adrenal medulla and their uptake into chromaffin granules" @default.
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- W2041205933 doi "https://doi.org/10.1016/0006-2952(76)90220-3" @default.
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