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- W2041222191 abstract "We show, using a simple, rapid fractionation method, that the precursor to the filamentous phage major coat protein is an integral membrane protein. The method, which consists of treatment of Escherichia coli with 0.1 N NaOH followed by centrifugation, leaves a subset of inner and outer membrane proteins in the NaOH pellet. Most proteins partition into the NaOH pellet (membrane) or supernatant (cytoplasm and periplasm) in a manner consistent with their subcellular location as determined by more conventional techniques. We find no evidence for cytoplasmic filamentous phage pre-coat protein in either wild-type of mutant-infected cells. Our evidence suggests that a protein identified as soluble procoat by K. Ito, G. Mandell and W. Wickner may be the amber fragment of a different phage protein." @default.
- W2041222191 created "2016-06-24" @default.
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- W2041222191 date "1982-01-01" @default.
- W2041222191 modified "2023-09-24" @default.
- W2041222191 title "Filamentous phage pre-coat is an integral membrane protein: Analysis by a new method of membrane preparation" @default.
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- W2041222191 doi "https://doi.org/10.1016/0092-8674(82)90387-7" @default.
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