Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041286657> ?p ?o ?g. }
- W2041286657 endingPage "e98921" @default.
- W2041286657 startingPage "e98921" @default.
- W2041286657 abstract "Grape seed procyanidins (PC) are flavan-3-ol oligomers and polymers known for their biological activity in the gut. Grape seed extract (GSE) have been reported to reduce intestinal injury in a rat model of mucositis. We sought to investigate effects of purified PC fractions differing in mean degree of polymerization (mDP) combined with 5-Fluorouracil (5-FU) chemotherapy on the viability of colon cancer cells (Caco-2).SixPC fractions (F1-F6) were isolated from Cabernet Sauvignon seeds at two ripeness stages: pre-veraison unripe (immature) and ripe (mature), utilizing step gradient, low-pressure chromatography on a Sephadex LH-20 resin. Fractions were tested on Caco-2 cells, alone and in combination with 5-FU. Eluted fractions were characterized by phloroglucinolysis and gel permeation chromatography. Cell viability was determined by the 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide) (MTT) assay.All isolated fractions significantly reduced Caco-2 cell viability compared to the control (P<0.05), but F2 and F3 (mDP 2-6) were the most active fractions (immature F2 = 32% mDP 2.4, F3 = 35% mDP 5.8 and mature F2 = 13% mDP 3.6 and F3 = 17% mDP 5.9; percentage of viable cells remaining) on Caco-2 cells. When combined with 5-FU, immature fractions F1-F3 enhanced the cell toxicity effects of 5-FU by 27-73% (P<0.05). Mature seed PC fractions (F1-F4) significantly enhanced the toxicity of 5-FU by 60-83% against Caco-2 cells (P<0.05). Moreover, some fractions alone were more potent at decreasing viability in Caco-2 cells (P<0.05; immature fractions = 65-68% and mature fractions = 83-87%) compared to 5-FU alone (37%).PCs of mDP 2-6 (immature F1-F3 and mature F1 and F4)not only enhanced the impact of 5-FU in killing Caco-2 cells, but also surpassed standard 5-FU chemotherapy as an anti-cancer agent.The bioactivity of PC is therefore attributed primarily to lower molecular weight PCs." @default.
- W2041286657 created "2016-06-24" @default.
- W2041286657 creator A5000542010 @default.
- W2041286657 creator A5002745126 @default.
- W2041286657 creator A5032555498 @default.
- W2041286657 creator A5073669425 @default.
- W2041286657 creator A5086539748 @default.
- W2041286657 date "2014-06-06" @default.
- W2041286657 modified "2023-10-16" @default.
- W2041286657 title "Low Molecular Weight Procyanidins from Grape Seeds Enhance the Impact of 5-Fluorouracil Chemotherapy on Caco-2 Human Colon Cancer Cells" @default.
- W2041286657 cites W1504463989 @default.
- W2041286657 cites W1967275637 @default.
- W2041286657 cites W1973253054 @default.
- W2041286657 cites W1980536033 @default.
- W2041286657 cites W1985234560 @default.
- W2041286657 cites W1985573684 @default.
- W2041286657 cites W1987350032 @default.
- W2041286657 cites W1988510047 @default.
- W2041286657 cites W1995865662 @default.
- W2041286657 cites W1999732755 @default.
- W2041286657 cites W2005379310 @default.
- W2041286657 cites W2005498160 @default.
- W2041286657 cites W2008226491 @default.
- W2041286657 cites W2017617644 @default.
- W2041286657 cites W2029367896 @default.
- W2041286657 cites W2031682879 @default.
- W2041286657 cites W2032521663 @default.
- W2041286657 cites W2034721665 @default.
- W2041286657 cites W2034945330 @default.
- W2041286657 cites W2040089109 @default.
- W2041286657 cites W2053232746 @default.
- W2041286657 cites W2060299576 @default.
- W2041286657 cites W2066193273 @default.
- W2041286657 cites W2070277360 @default.
- W2041286657 cites W2071613880 @default.
- W2041286657 cites W2076398648 @default.
- W2041286657 cites W2085822581 @default.
- W2041286657 cites W2086129491 @default.
- W2041286657 cites W2086959952 @default.
- W2041286657 cites W2092746415 @default.
- W2041286657 cites W2095096262 @default.
- W2041286657 cites W2141956082 @default.
- W2041286657 cites W2155281823 @default.
- W2041286657 cites W2158734599 @default.
- W2041286657 cites W2329782067 @default.
- W2041286657 doi "https://doi.org/10.1371/journal.pone.0098921" @default.
- W2041286657 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4048230" @default.
- W2041286657 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24905821" @default.
- W2041286657 hasPublicationYear "2014" @default.
- W2041286657 type Work @default.
- W2041286657 sameAs 2041286657 @default.
- W2041286657 citedByCount "41" @default.
- W2041286657 countsByYear W20412866572015 @default.
- W2041286657 countsByYear W20412866572016 @default.
- W2041286657 countsByYear W20412866572017 @default.
- W2041286657 countsByYear W20412866572018 @default.
- W2041286657 countsByYear W20412866572019 @default.
- W2041286657 countsByYear W20412866572020 @default.
- W2041286657 countsByYear W20412866572021 @default.
- W2041286657 countsByYear W20412866572022 @default.
- W2041286657 countsByYear W20412866572023 @default.
- W2041286657 crossrefType "journal-article" @default.
- W2041286657 hasAuthorship W2041286657A5000542010 @default.
- W2041286657 hasAuthorship W2041286657A5002745126 @default.
- W2041286657 hasAuthorship W2041286657A5032555498 @default.
- W2041286657 hasAuthorship W2041286657A5073669425 @default.
- W2041286657 hasAuthorship W2041286657A5086539748 @default.
- W2041286657 hasBestOaLocation W20412866571 @default.
- W2041286657 hasConcept C121608353 @default.
- W2041286657 hasConcept C142724271 @default.
- W2041286657 hasConcept C1491633281 @default.
- W2041286657 hasConcept C163994747 @default.
- W2041286657 hasConcept C178790620 @default.
- W2041286657 hasConcept C181199279 @default.
- W2041286657 hasConcept C185592680 @default.
- W2041286657 hasConcept C193176779 @default.
- W2041286657 hasConcept C204787440 @default.
- W2041286657 hasConcept C2776971262 @default.
- W2041286657 hasConcept C29730261 @default.
- W2041286657 hasConcept C31903555 @default.
- W2041286657 hasConcept C521977710 @default.
- W2041286657 hasConcept C526805850 @default.
- W2041286657 hasConcept C53227056 @default.
- W2041286657 hasConcept C54355233 @default.
- W2041286657 hasConcept C55493867 @default.
- W2041286657 hasConcept C63179538 @default.
- W2041286657 hasConcept C71924100 @default.
- W2041286657 hasConcept C86803240 @default.
- W2041286657 hasConceptScore W2041286657C121608353 @default.
- W2041286657 hasConceptScore W2041286657C142724271 @default.
- W2041286657 hasConceptScore W2041286657C1491633281 @default.
- W2041286657 hasConceptScore W2041286657C163994747 @default.
- W2041286657 hasConceptScore W2041286657C178790620 @default.
- W2041286657 hasConceptScore W2041286657C181199279 @default.
- W2041286657 hasConceptScore W2041286657C185592680 @default.
- W2041286657 hasConceptScore W2041286657C193176779 @default.
- W2041286657 hasConceptScore W2041286657C204787440 @default.
- W2041286657 hasConceptScore W2041286657C2776971262 @default.