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- W2041336105 abstract "Pigs from a variety of sources were surveyed for oro-gastrointestinal (oro-GIT) carriage of Candida albicans. Candida albicans-positive animals were readily located, but we also identified C. albicans-free pigs. We hypothesized that pigs could be stably colonized with a C. albicans strain of choice, simply by feeding yeast cells. Piglets were farrowed routinely and remained with the sow for 4 days to acquire a normal microbiota. Piglets were then placed in an artificial rearing environment and fed sow milk replacer. Piglets were inoculated orally with one of three different C. albicans strains. Piglets were weighed daily, and culture swabs were collected to detect C. albicans orally, rectally and in the piglet's environment. Stable C. albicans colonization over the course of the study did not affect piglet growth. Necropsy revealed mucosally associated C. albicans throughout the oro-GIT with the highest abundance in the esophagus. Uninoculated control piglets remained C. albicans-negative. These data establish the piglet as a model to study C. albicans colonization of the human oro-GIT. Similarities between oro-GIT colonization in humans and pigs, as well as the ease of working with the piglet model, suggest its adaptability for use among investigators interested in understanding C. albicans–host commensal interactions. Feeding yeast cells to normally farrowed piglets establishes stable colonization with Candida albicans, demonstrating the utility of the piglet as a model for the study of human C. albicans commensalism." @default.
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- W2041336105 date "2014-06-26" @default.
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- W2041336105 title "A piglet model for studying<i>Candida albicans</i>colonization of the human oro-gastrointestinal tract" @default.
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- W2041336105 doi "https://doi.org/10.1111/1574-6968.12500" @default.
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