Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041374008> ?p ?o ?g. }
- W2041374008 endingPage "859" @default.
- W2041374008 startingPage "853" @default.
- W2041374008 abstract "Previous reports have implicated connexin 43 (Cx43) as a tumor suppressor in early stages of tumorigenesis and in some cases as an enhancer of cell migration in later stages. To address the role of Cx43 in melanoma tumor progression, we utilized two melanoma cell lines derived from the same patient in pre-metastasis (WM793B) and following isolation from a lung metastasis in nude mice (1205Lu). Our results demonstrate a strikingly increased expression of Cx43 in both the pre-metastatic and metastatic melanoma cell lines that were actively migrating compared to non-migrating cells. To further investigate the role of Cx43 in these melanoma cells, we overexpressed wild type (wt) Cx43 as well as a mutant dominant negative Cx43 mutant that causes closed channels (T154A). The metastatic 1205Lu cells expressing Cx43-T154A showed a twofold decrease in colony formation on soft agar while the nonmetastatic WM793B cells showed no significant change. In invasion assays through a collagen matrix, the same Cx43-T154A 1205Lu cells demonstrated a three- to fourfold increase in the invasion index compared to either wt Cx43 or vector control cells. The increase in invasiveness was eliminated by migration towards media with charcoal-stripped serum, suggesting that migration may be directed towards a lipophilic compound(s). Our findings demonstrate that a dominant negative Cx43 mutant deficient in channel formation exhibits a dual pattern of regulation in metastatic melanoma cells with a decrease in anchorage-independent growth and an increase in invasive potential. J. Cell. Physiol. 228: 853–859, 2013. © 2012 Wiley Periodicals, Inc." @default.
- W2041374008 created "2016-06-24" @default.
- W2041374008 creator A5011131850 @default.
- W2041374008 creator A5018684446 @default.
- W2041374008 creator A5050382210 @default.
- W2041374008 creator A5056812960 @default.
- W2041374008 creator A5066379422 @default.
- W2041374008 creator A5083574626 @default.
- W2041374008 date "2012-12-20" @default.
- W2041374008 modified "2023-09-26" @default.
- W2041374008 title "A dominant negative Cx43 mutant differentially affects tumorigenic and invasive properties in human metastatic melanoma cells" @default.
- W2041374008 cites W1965070595 @default.
- W2041374008 cites W1984038646 @default.
- W2041374008 cites W1987594610 @default.
- W2041374008 cites W1988849665 @default.
- W2041374008 cites W1991914992 @default.
- W2041374008 cites W1992547890 @default.
- W2041374008 cites W2002379953 @default.
- W2041374008 cites W2019015469 @default.
- W2041374008 cites W2020294738 @default.
- W2041374008 cites W2023087233 @default.
- W2041374008 cites W2023992312 @default.
- W2041374008 cites W2024010922 @default.
- W2041374008 cites W2030092260 @default.
- W2041374008 cites W2035884066 @default.
- W2041374008 cites W2039732537 @default.
- W2041374008 cites W2060447264 @default.
- W2041374008 cites W2067043311 @default.
- W2041374008 cites W2077483652 @default.
- W2041374008 cites W2080321788 @default.
- W2041374008 cites W2080678747 @default.
- W2041374008 cites W2081454368 @default.
- W2041374008 cites W2083786873 @default.
- W2041374008 cites W2084527887 @default.
- W2041374008 cites W2090173312 @default.
- W2041374008 cites W2091853672 @default.
- W2041374008 cites W2096688825 @default.
- W2041374008 cites W2103779343 @default.
- W2041374008 cites W2110049914 @default.
- W2041374008 cites W2118450542 @default.
- W2041374008 cites W2119625199 @default.
- W2041374008 cites W2125951597 @default.
- W2041374008 cites W2127689320 @default.
- W2041374008 cites W2128945272 @default.
- W2041374008 cites W2134935836 @default.
- W2041374008 cites W2140769225 @default.
- W2041374008 cites W2141497573 @default.
- W2041374008 cites W2148188629 @default.
- W2041374008 cites W2152551864 @default.
- W2041374008 cites W2154911363 @default.
- W2041374008 cites W2171409673 @default.
- W2041374008 cites W4247211889 @default.
- W2041374008 doi "https://doi.org/10.1002/jcp.24235" @default.
- W2041374008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23042412" @default.
- W2041374008 hasPublicationYear "2012" @default.
- W2041374008 type Work @default.
- W2041374008 sameAs 2041374008 @default.
- W2041374008 citedByCount "23" @default.
- W2041374008 countsByYear W20413740082013 @default.
- W2041374008 countsByYear W20413740082014 @default.
- W2041374008 countsByYear W20413740082015 @default.
- W2041374008 countsByYear W20413740082016 @default.
- W2041374008 countsByYear W20413740082017 @default.
- W2041374008 countsByYear W20413740082019 @default.
- W2041374008 countsByYear W20413740082020 @default.
- W2041374008 countsByYear W20413740082021 @default.
- W2041374008 countsByYear W20413740082022 @default.
- W2041374008 countsByYear W20413740082023 @default.
- W2041374008 crossrefType "journal-article" @default.
- W2041374008 hasAuthorship W2041374008A5011131850 @default.
- W2041374008 hasAuthorship W2041374008A5018684446 @default.
- W2041374008 hasAuthorship W2041374008A5050382210 @default.
- W2041374008 hasAuthorship W2041374008A5056812960 @default.
- W2041374008 hasAuthorship W2041374008A5066379422 @default.
- W2041374008 hasAuthorship W2041374008A5083574626 @default.
- W2041374008 hasConcept C104317684 @default.
- W2041374008 hasConcept C121608353 @default.
- W2041374008 hasConcept C137738243 @default.
- W2041374008 hasConcept C143065580 @default.
- W2041374008 hasConcept C1491633281 @default.
- W2041374008 hasConcept C2777658100 @default.
- W2041374008 hasConcept C2779013556 @default.
- W2041374008 hasConcept C502942594 @default.
- W2041374008 hasConcept C54355233 @default.
- W2041374008 hasConcept C555283112 @default.
- W2041374008 hasConcept C62112901 @default.
- W2041374008 hasConcept C81885089 @default.
- W2041374008 hasConcept C86803240 @default.
- W2041374008 hasConceptScore W2041374008C104317684 @default.
- W2041374008 hasConceptScore W2041374008C121608353 @default.
- W2041374008 hasConceptScore W2041374008C137738243 @default.
- W2041374008 hasConceptScore W2041374008C143065580 @default.
- W2041374008 hasConceptScore W2041374008C1491633281 @default.
- W2041374008 hasConceptScore W2041374008C2777658100 @default.
- W2041374008 hasConceptScore W2041374008C2779013556 @default.
- W2041374008 hasConceptScore W2041374008C502942594 @default.
- W2041374008 hasConceptScore W2041374008C54355233 @default.
- W2041374008 hasConceptScore W2041374008C555283112 @default.