FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041388973> ?p ?o ?g. }

• W2041388973 endingPage "10" @default.
• W2041388973 startingPage "2" @default.
• W2041388973 abstract "Indirect evidence suggests that glucocorticoid hormones may act through cellular receptors to play a neuromodulatory role in the teleost CNS. We now report our findings on the use of [3H]dexamethasone (DEX) to identify hypothalamic glucocorticoid receptors (GRs) in juvenile rainbow trout, Oncorhynchus mykiss. Hypothalamic cytosol was incubated with [3H]DEX under various experimental paradigms with incubations terminated by addition of dextran-coated charcoal; following immediate centrifugation, a sample of bound [3H]DEX (supernatant) was collected and assessed for 3 H content. [3H]DEX binding was tissue dependent between 0.5 and 2. 0 hypothalamus equivalents per tube (1.0 to 4.7 mg protein, respectively). Specific binding (BSP) increased with time for 1.5 h and remained relatively constant for an additional 2.5 h; the calculated association rate constant was 2.23 x 10(8) M-1 x min-1. Equilibrium BSP was dissociated by addition of a 5000 M excess cortisol with an accompaning t1/2 of 1.25 h and dissociation rate constant of 0.553 min-1. BSP was saturable with a calculated equilibrium Kd and BMAX of 1.22 nM and 296 fmol/mg protein, respectively. BSP was displaced under equilibrium conditions by the corticosteroids, but to a lesser extent by the mineralocorticoid, estrogen, and progestin. The rank order of potency for [3H]DEX displacement was DEX > cortisol >> corticosterone > m triamcinolone = 11-deoxycortisol >> aldosterone > progesterone >>> 17 beta-estradiol. These properties of specifically bound [3H]DEX indicate the presence of a GR, similar to the mammalian cytosolic GR, in the hypothalamus of juvenile rainbow trout." @default.
• W2041388973 created "2016-06-24" @default.
• W2041388973 creator A5051313802 @default.
• W2041388973 creator A5081375351 @default.
• W2041388973 date "1998-04-01" @default.
• W2041388973 modified "2023-09-23" @default.
• W2041388973 title "Specific Binding Sites for [3H]Dexamethasone in the Hypothalamus of Juvenile Rainbow Trout,Oncorhynchus mykiss" @default.
• W2041388973 cites W1507028094 @default.
• W2041388973 cites W1965372040 @default.
• W2041388973 cites W1969222626 @default.
• W2041388973 cites W1972958136 @default.
• W2041388973 cites W1975877020 @default.
• W2041388973 cites W1983649348 @default.
• W2041388973 cites W1994358547 @default.
• W2041388973 cites W1994720086 @default.
• W2041388973 cites W1999135070 @default.
• W2041388973 cites W2005130856 @default.
• W2041388973 cites W2011244206 @default.
• W2041388973 cites W2015131453 @default.
• W2041388973 cites W2015738213 @default.
• W2041388973 cites W2017558508 @default.
• W2041388973 cites W2020911412 @default.
• W2041388973 cites W2027883508 @default.
• W2041388973 cites W2031159199 @default.
• W2041388973 cites W2055806024 @default.
• W2041388973 cites W2057163861 @default.
• W2041388973 cites W2061196213 @default.
• W2041388973 cites W2061243027 @default.
• W2041388973 cites W2065127351 @default.
• W2041388973 cites W2070066942 @default.
• W2041388973 cites W2070402931 @default.
• W2041388973 cites W2071070948 @default.
• W2041388973 cites W2079129489 @default.
• W2041388973 cites W2108264595 @default.
• W2041388973 cites W2140850665 @default.
• W2041388973 cites W2155721037 @default.
• W2041388973 cites W2164797945 @default.
• W2041388973 cites W2165471929 @default.
• W2041388973 cites W350327937 @default.
• W2041388973 cites W4293247451 @default.
• W2041388973 doi "https://doi.org/10.1006/gcen.1997.7043" @default.
• W2041388973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9514842" @default.
• W2041388973 hasPublicationYear "1998" @default.
• W2041388973 type Work @default.
• W2041388973 sameAs 2041388973 @default.
• W2041388973 citedByCount "22" @default.
• W2041388973 countsByYear W20413889732012 @default.
• W2041388973 countsByYear W20413889732013 @default.
• W2041388973 crossrefType "journal-article" @default.
• W2041388973 hasAuthorship W2041388973A5051313802 @default.
• W2041388973 hasAuthorship W2041388973A5081375351 @default.
• W2041388973 hasConcept C126322002 @default.
• W2041388973 hasConcept C134018914 @default.
• W2041388973 hasConcept C170493617 @default.
• W2041388973 hasConcept C181199279 @default.
• W2041388973 hasConcept C2776069600 @default.
• W2041388973 hasConcept C2777003273 @default.
• W2041388973 hasConcept C2778525890 @default.
• W2041388973 hasConcept C2780352252 @default.
• W2041388973 hasConcept C2780841215 @default.
• W2041388973 hasConcept C2909208804 @default.
• W2041388973 hasConcept C2994167347 @default.
• W2041388973 hasConcept C505870484 @default.
• W2041388973 hasConcept C55493867 @default.
• W2041388973 hasConcept C59493245 @default.
• W2041388973 hasConcept C71315377 @default.
• W2041388973 hasConcept C71924100 @default.
• W2041388973 hasConcept C74998103 @default.
• W2041388973 hasConcept C86803240 @default.
• W2041388973 hasConcept C98539663 @default.
• W2041388973 hasConceptScore W2041388973C126322002 @default.
• W2041388973 hasConceptScore W2041388973C134018914 @default.
• W2041388973 hasConceptScore W2041388973C170493617 @default.
• W2041388973 hasConceptScore W2041388973C181199279 @default.
• W2041388973 hasConceptScore W2041388973C2776069600 @default.
• W2041388973 hasConceptScore W2041388973C2777003273 @default.
• W2041388973 hasConceptScore W2041388973C2778525890 @default.
• W2041388973 hasConceptScore W2041388973C2780352252 @default.
• W2041388973 hasConceptScore W2041388973C2780841215 @default.
• W2041388973 hasConceptScore W2041388973C2909208804 @default.
• W2041388973 hasConceptScore W2041388973C2994167347 @default.
• W2041388973 hasConceptScore W2041388973C505870484 @default.
• W2041388973 hasConceptScore W2041388973C55493867 @default.
• W2041388973 hasConceptScore W2041388973C59493245 @default.
• W2041388973 hasConceptScore W2041388973C71315377 @default.
• W2041388973 hasConceptScore W2041388973C71924100 @default.
• W2041388973 hasConceptScore W2041388973C74998103 @default.
• W2041388973 hasConceptScore W2041388973C86803240 @default.
• W2041388973 hasConceptScore W2041388973C98539663 @default.
• W2041388973 hasIssue "1" @default.
• W2041388973 hasLocation W20413889731 @default.
• W2041388973 hasLocation W20413889732 @default.
• W2041388973 hasOpenAccess W2041388973 @default.
• W2041388973 hasPrimaryLocation W20413889731 @default.
• W2041388973 hasRelatedWork W1977153383 @default.
• W2041388973 hasRelatedWork W2016322361 @default.
• W2041388973 hasRelatedWork W2034583712 @default.
• W2041388973 hasRelatedWork W2044827976 @default.

• next