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- W2041398685 abstract "Four commercially available immunocytochemistry kits were compared for their ability to detect human cytomegalovirus (HCMV) in tissue culture and histologic sections using HCMV specific murine monoclonal antibodies. The procedures evaluated included two avidin-biotin complex (ABC) kits with either alkaline phosphatase or peroxidase coupled to biotin, an indirect immunogold silver enhancement (IGSS) kit using goat-antimouse antibody-5 nm gold sol, and a streptavidin- 5 nm gold sol kit. For HCMV detection in tissue culture, the ABC-alkaline phosphatase kit was superior to the ABCperoxidase and indirect IGSS kits. The streptavidin IGSS kit was also superior to the indirect IGSS kit in its ability to detect HCMV in tissue culture. Of the three silver enhancement techniques, the IntenSE I1 provided the best balance between ease of administration and intensity of staining. The streptavidin IGSS kit performed well in demonstrating HCMV-infected cells in histologic sections. These studies demonstrate that streptavidin IGSS is a useful immunocytochemical procedure that can be used for studies involving HCMV diagnoses, and HCMV pathogenetic studies. (The J Histotechnol 12:9, 1989)." @default.
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- W2041398685 date "1989-03-01" @default.
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- W2041398685 title "Detection of Human Cytomegalovirus by Immunogold and Silver Enhancement" @default.
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- W2041398685 doi "https://doi.org/10.1179/his.1989.12.1.9" @default.
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