FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041419642> ?p ?o ?g. }

• W2041419642 abstract "AcrAB-TolC and their homologs are major multidrug efflux systems in Gram-negative bacteria. The inner membrane component AcrB functions as a trimer. Replacement of Pro223 by Gly in AcrB decreases the trimer stability and drastically reduces the drug efflux activity. The goal of this study is to identify suppressor mutations that restore function to mutant AcrBP223G and explore the mechanism of function recovery. Two methods were used to introduce random mutations into the plasmid of AcrBP223G. Mutants with elevated drug efflux activity were identified, purified, and characterized to examine their expression level, trimer stability, interaction with AcrA, and substrate binding. Nine single-site repressor mutations were identified, including T199M, D256N, A209V, G257V, M662I, Q737L, D788K, P800S, and E810K. Except for M662I, all other mutations located in the docking region of the periplasmic domain. While three mutations, T199M, A209V, and D256N, significantly increased the trimer stability, none of them restored the trimer affinity to the wild type level. M662, the only site of mutation that located in the porter domain, was involved in substrate binding. Our results suggest that the function loss resulted from compromised AcrB trimerization could be restored through various mechanisms involving the compensation of trimer stability and substrate binding." @default.
• W2041419642 created "2016-06-24" @default.
• W2041419642 creator A5007950824 @default.
• W2041419642 creator A5009578270 @default.
• W2041419642 creator A5031469247 @default.
• W2041419642 creator A5056309003 @default.
• W2041419642 creator A5062699888 @default.
• W2041419642 date "2015-01-22" @default.
• W2041419642 modified "2023-10-16" @default.
• W2041419642 title "Repressive mutations restore function-loss caused by the disruption of trimerization in Escherichia coli multidrug transporter AcrB" @default.
• W2041419642 cites W1785018154 @default.
• W2041419642 cites W1948806874 @default.
• W2041419642 cites W1964509770 @default.
• W2041419642 cites W1968140197 @default.
• W2041419642 cites W1973714359 @default.
• W2041419642 cites W1979696443 @default.
• W2041419642 cites W1985400607 @default.
• W2041419642 cites W1997870466 @default.
• W2041419642 cites W2010438788 @default.
• W2041419642 cites W2013251630 @default.
• W2041419642 cites W2013714352 @default.
• W2041419642 cites W2018396099 @default.
• W2041419642 cites W2019509230 @default.
• W2041419642 cites W2023463714 @default.
• W2041419642 cites W2026073246 @default.
• W2041419642 cites W2026437276 @default.
• W2041419642 cites W2028787442 @default.
• W2041419642 cites W2041681452 @default.
• W2041419642 cites W2049862828 @default.
• W2041419642 cites W2054248955 @default.
• W2041419642 cites W2056659809 @default.
• W2041419642 cites W2068119233 @default.
• W2041419642 cites W2071083537 @default.
• W2041419642 cites W2077534797 @default.
• W2041419642 cites W2077815188 @default.
• W2041419642 cites W2084778413 @default.
• W2041419642 cites W2096172849 @default.
• W2041419642 cites W2099540110 @default.
• W2041419642 cites W2108399347 @default.
• W2041419642 cites W2116300112 @default.
• W2041419642 cites W2120772351 @default.
• W2041419642 cites W2122646377 @default.
• W2041419642 cites W2132067013 @default.
• W2041419642 cites W2134483159 @default.
• W2041419642 cites W2138857675 @default.
• W2041419642 cites W2142318169 @default.
• W2041419642 cites W2150015383 @default.
• W2041419642 cites W2153601116 @default.
• W2041419642 cites W2155147384 @default.
• W2041419642 cites W2158530003 @default.
• W2041419642 cites W2328752440 @default.
• W2041419642 doi "https://doi.org/10.3389/fmicb.2015.00004" @default.
• W2041419642 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4303003" @default.
• W2041419642 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25657644" @default.
• W2041419642 hasPublicationYear "2015" @default.
• W2041419642 type Work @default.
• W2041419642 sameAs 2041419642 @default.
• W2041419642 citedByCount "7" @default.
• W2041419642 countsByYear W20414196422016 @default.
• W2041419642 countsByYear W20414196422017 @default.
• W2041419642 countsByYear W20414196422020 @default.
• W2041419642 countsByYear W20414196422021 @default.
• W2041419642 countsByYear W20414196422022 @default.
• W2041419642 countsByYear W20414196422023 @default.
• W2041419642 crossrefType "journal-article" @default.
• W2041419642 hasAuthorship W2041419642A5007950824 @default.
• W2041419642 hasAuthorship W2041419642A5009578270 @default.
• W2041419642 hasAuthorship W2041419642A5031469247 @default.
• W2041419642 hasAuthorship W2041419642A5056309003 @default.
• W2041419642 hasAuthorship W2041419642A5062699888 @default.
• W2041419642 hasBestOaLocation W20414196421 @default.
• W2041419642 hasConcept C104317684 @default.
• W2041419642 hasConcept C107824862 @default.
• W2041419642 hasConcept C115335371 @default.
• W2041419642 hasConcept C12554922 @default.
• W2041419642 hasConcept C143065580 @default.
• W2041419642 hasConcept C149011108 @default.
• W2041419642 hasConcept C178790620 @default.
• W2041419642 hasConcept C185592680 @default.
• W2041419642 hasConcept C200082930 @default.
• W2041419642 hasConcept C201663137 @default.
• W2041419642 hasConcept C2776403692 @default.
• W2041419642 hasConcept C2779546866 @default.
• W2041419642 hasConcept C501734568 @default.
• W2041419642 hasConcept C547475151 @default.
• W2041419642 hasConcept C55493867 @default.
• W2041419642 hasConcept C86803240 @default.
• W2041419642 hasConcept C95444343 @default.
• W2041419642 hasConceptScore W2041419642C104317684 @default.
• W2041419642 hasConceptScore W2041419642C107824862 @default.
• W2041419642 hasConceptScore W2041419642C115335371 @default.
• W2041419642 hasConceptScore W2041419642C12554922 @default.
• W2041419642 hasConceptScore W2041419642C143065580 @default.
• W2041419642 hasConceptScore W2041419642C149011108 @default.
• W2041419642 hasConceptScore W2041419642C178790620 @default.
• W2041419642 hasConceptScore W2041419642C185592680 @default.
• W2041419642 hasConceptScore W2041419642C200082930 @default.
• W2041419642 hasConceptScore W2041419642C201663137 @default.
• W2041419642 hasConceptScore W2041419642C2776403692 @default.
• W2041419642 hasConceptScore W2041419642C2779546866 @default.

• next