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- W2041444353 abstract "An amperometric biosensor was fabricated for the quantitative determination of urea in aqueous medium using hematein, a pH-sensitive natural dye. The urease (Urs) was covalently immobilized onto an electrode made of gold nanoparticles functionalized with hyperbranched polyester-Boltron® H40 (H40–Au) coated onto an indium–tin oxide (ITO) covered glass substrate. The covalent linkage between the Urs enzyme and H40–Au nanoparticles provided the resulting enzyme electrode (Urs/H40–Au/ITO) with a high level of enzyme immobilization and excellent lifetime stability. The response studies were carried out as a function of urea concentration with amperometric and photometric measurements. The biosensor based on Urs/H40–Au/ITO as the working electrode showed a linear current response to the urea concentration ranging from 0.01 to 35 mM. The urea biosensor exhibited a sensitivity of 7.48 nA/mM with a response time of 3 s. The Michaelis–Menten constant for the Urs/H40–Au/ITO biosensor was calculated to be 0.96 mM, indicating the Urs enzyme immobilized on the electrode surface had a high affinity to urea." @default.
- W2041444353 created "2016-06-24" @default.
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- W2041444353 date "2009-06-15" @default.
- W2041444353 modified "2023-10-11" @default.
- W2041444353 title "An amperometric urea biosensor based on covalently immobilized urease on an electrode made of hyperbranched polyester functionalized gold nanoparticles" @default.
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- W2041444353 doi "https://doi.org/10.1016/j.talanta.2009.02.038" @default.
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