Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041490761> ?p ?o ?g. }
- W2041490761 endingPage "3642" @default.
- W2041490761 startingPage "3633" @default.
- W2041490761 abstract "The human fibrosarcoma cell line, HT1080, clone H4, was used to determine if the transformation suppressive functions of p53 and Egr-1 have the same underlying mechanism. This cell line expresses only mutant p53 and no detectable Egr-1. H4 clones stably expressing Egr-1 are less transformed in proportion to the level of Egr-1 expressed, acting through the induction of the TGFβ1 gene. Here, H4 cells and the highest Egr-1 expressing clone were transfected with a vector expressing normal human p53 to derive stable clones expressing p53. The expression of p53 in H4 cells inhibited transformed growth and reduced tumorigenicity. The effect of co-expression of both p53 and Egr-1 was additive, producing cell lines with 30% of normal growth rate and sevenfold reduced tumorigenicity compared with control lines. These results indicated that each factor may act independently by different pathways, although each additively increased the level of p21WAF1 cell cycle inhibitor. However, exposure of the H4-derived cells to UV-C irradiation produced contrasting effects. Cell cycle analyses showed that the presence of p53 was associated with loss of the G1 and S cells to apoptosis after irradiation. In contrast, the expression of Egr-1 increased entry into S/G2 phase of the cell cycle with little apoptosis via a mechanism involving elevated FAK and low caspase activities. Apoptosis was observed only in the cell lines that expressed no Egr-1, especially those expressing wt-p53, and was preceded by high caspase activity. In summary, Egr-1 suppressed transformation and counteracted apoptosis by the coordinated activation of TGFβ1, FN, p21 and FAK, leading to enhanced cell attachment and reduced caspase activity. In the doubly expressing cell line, the survival effect of Egr-1 was dominant over the apoptotic effect of p53." @default.
- W2041490761 created "2016-06-24" @default.
- W2041490761 creator A5005190349 @default.
- W2041490761 creator A5011812220 @default.
- W2041490761 creator A5051583340 @default.
- W2041490761 creator A5077630778 @default.
- W2041490761 creator A5078875479 @default.
- W2041490761 creator A5081883286 @default.
- W2041490761 date "1999-06-17" @default.
- W2041490761 modified "2023-09-30" @default.
- W2041490761 title "p53 and Egr-1 additively suppress transformed growth in HT1080 cells but Egr-1 counteracts p53-dependent apoptosis" @default.
- W2041490761 cites W1495048402 @default.
- W2041490761 cites W1557686543 @default.
- W2041490761 cites W1964203341 @default.
- W2041490761 cites W1969943605 @default.
- W2041490761 cites W1972376460 @default.
- W2041490761 cites W1984874586 @default.
- W2041490761 cites W1986100686 @default.
- W2041490761 cites W1989780564 @default.
- W2041490761 cites W1991494214 @default.
- W2041490761 cites W1993004730 @default.
- W2041490761 cites W2002050823 @default.
- W2041490761 cites W2008780319 @default.
- W2041490761 cites W2014928186 @default.
- W2041490761 cites W2015519364 @default.
- W2041490761 cites W2017597379 @default.
- W2041490761 cites W2027923032 @default.
- W2041490761 cites W2029524486 @default.
- W2041490761 cites W2030848654 @default.
- W2041490761 cites W2034459218 @default.
- W2041490761 cites W2039462089 @default.
- W2041490761 cites W2052885484 @default.
- W2041490761 cites W2058561774 @default.
- W2041490761 cites W2060870231 @default.
- W2041490761 cites W2067762187 @default.
- W2041490761 cites W2077880395 @default.
- W2041490761 cites W2078344309 @default.
- W2041490761 cites W2079414703 @default.
- W2041490761 cites W2079917786 @default.
- W2041490761 cites W2085485098 @default.
- W2041490761 cites W2090025028 @default.
- W2041490761 cites W2090585013 @default.
- W2041490761 cites W2092181236 @default.
- W2041490761 cites W2092368761 @default.
- W2041490761 cites W2111986039 @default.
- W2041490761 cites W2113370863 @default.
- W2041490761 cites W2119222825 @default.
- W2041490761 cites W2122543608 @default.
- W2041490761 cites W2137022580 @default.
- W2041490761 cites W2148175319 @default.
- W2041490761 cites W2159235132 @default.
- W2041490761 cites W2159695799 @default.
- W2041490761 cites W2164111911 @default.
- W2041490761 cites W2167604659 @default.
- W2041490761 cites W2323995323 @default.
- W2041490761 cites W2407488403 @default.
- W2041490761 cites W4232548679 @default.
- W2041490761 doi "https://doi.org/10.1038/sj.onc.1202696" @default.
- W2041490761 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10380885" @default.
- W2041490761 hasPublicationYear "1999" @default.
- W2041490761 type Work @default.
- W2041490761 sameAs 2041490761 @default.
- W2041490761 citedByCount "85" @default.
- W2041490761 countsByYear W20414907612012 @default.
- W2041490761 countsByYear W20414907612013 @default.
- W2041490761 countsByYear W20414907612014 @default.
- W2041490761 countsByYear W20414907612015 @default.
- W2041490761 countsByYear W20414907612016 @default.
- W2041490761 countsByYear W20414907612017 @default.
- W2041490761 countsByYear W20414907612019 @default.
- W2041490761 countsByYear W20414907612021 @default.
- W2041490761 countsByYear W20414907612022 @default.
- W2041490761 countsByYear W20414907612023 @default.
- W2041490761 crossrefType "journal-article" @default.
- W2041490761 hasAuthorship W2041490761A5005190349 @default.
- W2041490761 hasAuthorship W2041490761A5011812220 @default.
- W2041490761 hasAuthorship W2041490761A5051583340 @default.
- W2041490761 hasAuthorship W2041490761A5077630778 @default.
- W2041490761 hasAuthorship W2041490761A5078875479 @default.
- W2041490761 hasAuthorship W2041490761A5081883286 @default.
- W2041490761 hasBestOaLocation W20414907611 @default.
- W2041490761 hasConcept C104317684 @default.
- W2041490761 hasConcept C105696609 @default.
- W2041490761 hasConcept C153911025 @default.
- W2041490761 hasConcept C190283241 @default.
- W2041490761 hasConcept C2776495794 @default.
- W2041490761 hasConcept C2777751087 @default.
- W2041490761 hasConcept C29537977 @default.
- W2041490761 hasConcept C502942594 @default.
- W2041490761 hasConcept C54009773 @default.
- W2041490761 hasConcept C54355233 @default.
- W2041490761 hasConcept C62112901 @default.
- W2041490761 hasConcept C81089528 @default.
- W2041490761 hasConcept C81885089 @default.
- W2041490761 hasConcept C86803240 @default.
- W2041490761 hasConcept C95444343 @default.
- W2041490761 hasConceptScore W2041490761C104317684 @default.
- W2041490761 hasConceptScore W2041490761C105696609 @default.