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• W2041501487 abstract "Transcription from the Escherichia coli rrnB P1 promoter is increased by a cis-acting sequence which extends upstream of the − 35 hexamer to about −150 with respect to the transcription initiation site, the Upstream Activation Region (UAR). Activation by the UAR involves two components: (1) a trans-acting protein, Fis, which binds to three sites in the UAR between −60 and −150, and (2) the UAR sequences themselves which affect RNA polymerase (RNAP) activity Independent of other proteins. We refer to the latter as Factor-Independent Activation (FIA). In addition to its interactions with the −10 and −35 hexamers typical of E. coll promoters, RNAP makes contacts to the − 53 region of rrnB P1, which may be related to the FIA effect. We constructed a series of insertion mutants containing Integral and non-integral numbers of helical turns at position −46, between the Fis binding sites and the − 35 region, and the resulting promoter activities were measured in vitro and In vivo. The data suggest that both Fis-dependent and factor-independent activation are face of the helix dependent: the Fis binding site and the sequences responsible for factor-independent activation must be correctly oriented relative to RNA polymerase in order to activate transcription. These results, in conjunction with other evidence, support a model for the Involvement of direct Fis-RNAP interactions in upstream activation. We also demonstrate that RNAP interacts with the − 53 region of the rrnB P1 UAR even when these sequences are displaced upstream of the RNAP binding site, and that these interactions correlate with factor-independent activation." @default.
• W2041501487 created "2016-06-24" @default.
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• W2041501487 date "1992-01-01" @default.
• W2041501487 modified "2023-09-26" @default.
• W2041501487 title "Both fis-dependent and factor-independent upstream activation of the rrnB P1 promoter are face of the helix dependent" @default.
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• W2041501487 doi "https://doi.org/10.1093/nar/20.4.719" @default.
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