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- W2041562662 abstract "A fucosyltransferase activity has been detected using lacto-N-biose I as acceptor in the lower eukaryote Dictyostelium discoideum. This transferase requires divalent cations and is inhibited by N-ethylmaleimide and detergent treatment. Apparent calculated Km values for GDP-Fuc and lacto-N-biose I are 1.27 microM and 2.80 mM, respectively. The activity is quantitatively recovered in the supernatant after centrifugation at 100000 x g for 1 h. The reaction product, as determined by gel permeation chromatography, sensitivity to fucosidases, and analysis of partially methylated derivatives, is Fucalpha1-2Galbeta1-3GlcNAc (H type 1 trisaccharide)." @default.
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- W2041562662 date "1996-10-14" @default.
- W2041562662 modified "2023-10-16" @default.
- W2041562662 title "Dictyosteliumcytosolic fucosyltransferase synthesizes H type 1 trisaccharide in vitro" @default.
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- W2041562662 doi "https://doi.org/10.1016/0014-5793(96)01003-4" @default.
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