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• W2041562819 startingPage "977" @default.
• W2041562819 abstract "In this article we report the first results on the enzymatic activity of horseradish peroxidase (HRP) microencapsulated in water-in-ionic liquid (w/IL) microemulsions using pyrogallol as the substrate. Toward this goal, the system used in this study was composed of anionic surfactant AOT (sodium bis(2-ethyl-1-hexyl)sulfosuccinate)/hydrophobic IL [C(8)mim][Tf(2)N] (1-octyl-3-methyl imidazolium bis(trifluoromethylsulfonyl)amide)/water/1-hexanol. In this system, the catalytic activity of HRP was measured as a function of substrate concentrations, W(0) (molar ratio of water to surfactant), pH, and 1-hexanol content. The curve of the activity-W(0) profile was found to be hyperbolic for the new microemulsion. The apparent Michaelis-Menten kinetic parameters (k(cat) and K(m)) were estimated and compared to those obtained from a conventional microemulsion. Apparently, it was found that HRP-catalyzed oxidation of pyrogallol by hydrogen peroxide in IL microemulsuions is much more effective than in a conventional AOT/water/isooctane microemulsion. The stability of HRP solubilized in the newly developed w/IL microemulsions was examined, and it was found that HRP retained almost 70% of its initial activity after incubation at 28 degrees C for 30 h." @default.
• W2041562819 created "2016-06-24" @default.
• W2041562819 creator A5014271929 @default.
• W2041562819 creator A5037912117 @default.
• W2041562819 creator A5072721448 @default.
• W2041562819 date "2008-12-29" @default.
• W2041562819 modified "2023-10-01" @default.
• W2041562819 title "Biocatalysis in Water-in-Ionic Liquid Microemulsions: A Case Study with Horseradish Peroxidase" @default.
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• W2041562819 doi "https://doi.org/10.1021/la803118q" @default.
• W2041562819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19113810" @default.
• W2041562819 hasPublicationYear "2008" @default.
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