FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041585304> ?p ?o ?g. }

• W2041585304 endingPage "2082" @default.
• W2041585304 startingPage "2072" @default.
• W2041585304 abstract "ABSTRACT Pseudomonas aeruginosa uses N -acyl-homoserine lactone (AHL)-dependent quorum sensing (QS) systems to control the expression of secreted effectors. These effectors can be crucial to the ecological fitness of the bacterium, playing roles in nutrient acquisition, microbial competition, and virulence. In this study, we investigated the metabolic consequences of AHL-dependent QS by monitoring the metabolic profile(s) of a lasI rhlI double mutant (unable to make QS signaling molecules) and its wild-type progenitor as they progressed through the growth curve. Analysis of culture supernatants by 1 H-nuclear magnetic resonance ( 1 H-NMR) spectroscopy revealed that at the point where AHL concentrations peaked in the wild type, the metabolic footprints (i.e., extracellular metabolites) of the wild-type and lasI rhlI mutant diverged. Subsequent gas chromatography-mass spectrometry (GC-MS)-based analysis of the intracellular metabolome revealed QS-dependent perturbations in around one-third of all identified metabolites, including altered concentrations of tricarboxylic acid (TCA) cycle intermediates, amino acids, and fatty acids. Further targeted fatty acid methyl ester (FAME) GC-MS-based profiling of the cellular total fatty acid pools revealed that QS leads to changes associated with decreased membrane fluidity and higher chemical stability. However, not all of the changes we observed were necessarily a direct consequence of QS; liquid chromatography (LC)-MS analyses revealed that polyamine levels were elevated in the lasI rhlI mutant, perhaps a response to the absence of QS-dependent adaptations. Our data suggest that QS leads to a global readjustment in central metabolism and provide new insight into the metabolic changes associated with QS during stationary-phase adaptation. IMPORTANCE Quorum sensing (QS) is a transcriptional regulatory mechanism that allows bacteria to coordinate their gene expression profile with the population cell density. The opportunistic human pathogen Pseudomonas aeruginosa uses QS to control the production of secreted virulence factors. In this study, we show that QS elicits a global “metabolic rewiring” in P. aeruginosa . This metabolic rerouting of fluxes is consistent with a variety of drivers, ranging from altered QS-dependent transcription of “metabolic genes” through to the effect(s) of global “metabolic readjustment” as a consequence of QS-dependent exoproduct synthesis, as well as a general stress response, among others. To our knowledge, this is the first study of its kind to assess the global impact of QS on the metabolome." @default.
• W2041585304 created "2016-06-24" @default.
• W2041585304 creator A5057438504 @default.
• W2041585304 creator A5058889907 @default.
• W2041585304 creator A5076795620 @default.
• W2041585304 date "2015-06-15" @default.
• W2041585304 modified "2023-10-11" @default.
• W2041585304 title "Quorum Sensing Is Accompanied by Global Metabolic Changes in the Opportunistic Human Pathogen Pseudomonas aeruginosa" @default.
• W2041585304 cites W1479878391 @default.
• W2041585304 cites W1532082124 @default.
• W2041585304 cites W1555500408 @default.
• W2041585304 cites W1803682227 @default.
• W2041585304 cites W1863760789 @default.
• W2041585304 cites W1964466122 @default.
• W2041585304 cites W1967221794 @default.
• W2041585304 cites W1972252150 @default.
• W2041585304 cites W1979400645 @default.
• W2041585304 cites W1979888857 @default.
• W2041585304 cites W1980352067 @default.
• W2041585304 cites W1983495015 @default.
• W2041585304 cites W1993381520 @default.
• W2041585304 cites W1993562651 @default.
• W2041585304 cites W1993729354 @default.
• W2041585304 cites W1998409894 @default.
• W2041585304 cites W2006061929 @default.
• W2041585304 cites W2006311044 @default.
• W2041585304 cites W2010912096 @default.
• W2041585304 cites W2015234711 @default.
• W2041585304 cites W2015718294 @default.
• W2041585304 cites W2023517595 @default.
• W2041585304 cites W2023825654 @default.
• W2041585304 cites W2027229485 @default.
• W2041585304 cites W2034129786 @default.
• W2041585304 cites W2052043623 @default.
• W2041585304 cites W2056157585 @default.
• W2041585304 cites W2058225831 @default.
• W2041585304 cites W2060839079 @default.
• W2041585304 cites W2083761792 @default.
• W2041585304 cites W2086358238 @default.
• W2041585304 cites W2087371604 @default.
• W2041585304 cites W2087668809 @default.
• W2041585304 cites W2091078375 @default.
• W2041585304 cites W2092550686 @default.
• W2041585304 cites W2096861885 @default.
• W2041585304 cites W2097944090 @default.
• W2041585304 cites W2099749277 @default.
• W2041585304 cites W2103697673 @default.
• W2041585304 cites W2103780237 @default.
• W2041585304 cites W2106663365 @default.
• W2041585304 cites W2112814507 @default.
• W2041585304 cites W2113439641 @default.
• W2041585304 cites W2115407301 @default.
• W2041585304 cites W2118260061 @default.
• W2041585304 cites W2122955128 @default.
• W2041585304 cites W2125021619 @default.
• W2041585304 cites W2130003824 @default.
• W2041585304 cites W2136847512 @default.
• W2041585304 cites W2136918853 @default.
• W2041585304 cites W2137215017 @default.
• W2041585304 cites W2138785360 @default.
• W2041585304 cites W2142758007 @default.
• W2041585304 cites W2146292204 @default.
• W2041585304 cites W2148054572 @default.
• W2041585304 cites W2150967287 @default.
• W2041585304 cites W2152875113 @default.
• W2041585304 cites W2153883021 @default.
• W2041585304 cites W2154043802 @default.
• W2041585304 cites W2154970061 @default.
• W2041585304 cites W2163806992 @default.
• W2041585304 cites W2166816492 @default.
• W2041585304 cites W2168337709 @default.
• W2041585304 cites W2169415577 @default.
• W2041585304 cites W2171527366 @default.
• W2041585304 cites W2527496132 @default.
• W2041585304 cites W2951807980 @default.
• W2041585304 doi "https://doi.org/10.1128/jb.02557-14" @default.
• W2041585304 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4438216" @default.
• W2041585304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25868647" @default.
• W2041585304 hasPublicationYear "2015" @default.
• W2041585304 type Work @default.
• W2041585304 sameAs 2041585304 @default.
• W2041585304 citedByCount "65" @default.
• W2041585304 countsByYear W20415853042015 @default.
• W2041585304 countsByYear W20415853042016 @default.
• W2041585304 countsByYear W20415853042017 @default.
• W2041585304 countsByYear W20415853042018 @default.
• W2041585304 countsByYear W20415853042019 @default.
• W2041585304 countsByYear W20415853042020 @default.
• W2041585304 countsByYear W20415853042021 @default.
• W2041585304 countsByYear W20415853042022 @default.
• W2041585304 countsByYear W20415853042023 @default.
• W2041585304 crossrefType "journal-article" @default.
• W2041585304 hasAuthorship W2041585304A5057438504 @default.
• W2041585304 hasAuthorship W2041585304A5058889907 @default.
• W2041585304 hasAuthorship W2041585304A5076795620 @default.
• W2041585304 hasBestOaLocation W20415853041 @default.
• W2041585304 hasConcept C104317684 @default.
• W2041585304 hasConcept C118687296 @default.

• next