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- W2041752681 abstract "Chromatographic separation of amino acids with fluorometric detection of the o-phthalaldehyde-thiol reaction product offers an analytical system of high sensitivity for most amino acids. The fluorescence yield for amino acids having a fully substituted carbon atom in the α-position with respect to the amino group can be substantially improved by increasing the temperature of the reaction with o-phthalaldehyde-thiol and by substituting either ethanethiol or methanethiol for the more commonly used β-mercaptoethanol. The analytical sensitivity for these α-branched amino acids is thus brought into line with that for the other primary amino acids. A comparison of chromatograms obtained at reaction temperatures of 25 and 100°C allows recognition of amino acids of this type in complex mixtures by the substantial increase in fluorescence yield at 100°C." @default.
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- W2041752681 date "1979-01-01" @default.
- W2041752681 modified "2023-09-26" @default.
- W2041752681 title "Amino acid analysis with o-phthalaldehyde detection: Effects of reaction temperature and thiol on fluorescence yields" @default.
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- W2041752681 doi "https://doi.org/10.1016/s0003-2697(79)80133-5" @default.
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