Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041773087> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2041773087 abstract "Tetrahymena vorax (T. vorax) is an indigenous fresh water protozoan with the natural biological potential to maintain a specific aquatic microbial flora by ingesting and eliminating specific microorganism. To investigate the molecular mechanisms controlling Tetrahymena vorax (T. vorax) cellular differentiation from a small-mouth vegetative cell to a voracious large-mouth carnivore capable of ingesting prey ciliates and bacteria from aquatic environments, we use DNA subtraction and gene discovery techniques to identify and isolate T. vorax differentiation-specific genes. The physiological necessity for one newly discovered gene, SUBII-TG, was determined in vivo using an antisense oligonucleotide directed against the 5' SUBII-TG DNA sequence. The barriers to delivering antisense oligonucleotides to the cytoplasm of T. vorax were circumvented by employing a new but simple procedure of processing the oligonucleotide with the differentiation stimulus, stomatin. In these studies, the antisense oligonucleotide down-regulated SUBII-TG mRNA expression, and blocked differentiation and ingestion of prey ciliates. The ability to down-regulate SUBII-TG expression with the antisense oligonucleotide suggests that the molecular mechanisms controlling the natural biological activities of T. vorax can be manipulated to further study its cellular differentiation and potential as a biocontrol microorganism." @default.
- W2041773087 created "2016-06-24" @default.
- W2041773087 creator A5010554449 @default.
- W2041773087 creator A5024351490 @default.
- W2041773087 creator A5079125985 @default.
- W2041773087 date "2000-12-01" @default.
- W2041773087 modified "2023-09-27" @default.
- W2041773087 title "Biological and molecular characterization of cellular differentiation inTetrahymena vorax: A potential biocontrol protozoan" @default.
- W2041773087 cites W1526839960 @default.
- W2041773087 cites W1971670042 @default.
- W2041773087 cites W1975304761 @default.
- W2041773087 cites W197531747 @default.
- W2041773087 cites W2007319015 @default.
- W2041773087 cites W2028622989 @default.
- W2041773087 cites W2033666011 @default.
- W2041773087 cites W2047843046 @default.
- W2041773087 cites W2050057842 @default.
- W2041773087 cites W2054800842 @default.
- W2041773087 cites W2056061721 @default.
- W2041773087 cites W2060333964 @default.
- W2041773087 cites W2114786802 @default.
- W2041773087 cites W2142339245 @default.
- W2041773087 cites W2144112248 @default.
- W2041773087 cites W2799478281 @default.
- W2041773087 doi "https://doi.org/10.1002/1521-4028(200012)40:5/6<351::aid-jobm351>3.0.co;2-q" @default.
- W2041773087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11199495" @default.
- W2041773087 hasPublicationYear "2000" @default.
- W2041773087 type Work @default.
- W2041773087 sameAs 2041773087 @default.
- W2041773087 citedByCount "2" @default.
- W2041773087 crossrefType "journal-article" @default.
- W2041773087 hasAuthorship W2041773087A5010554449 @default.
- W2041773087 hasAuthorship W2041773087A5024351490 @default.
- W2041773087 hasAuthorship W2041773087A5079125985 @default.
- W2041773087 hasConcept C104317684 @default.
- W2041773087 hasConcept C129312508 @default.
- W2041773087 hasConcept C2777166614 @default.
- W2041773087 hasConcept C552990157 @default.
- W2041773087 hasConcept C55493867 @default.
- W2041773087 hasConcept C86803240 @default.
- W2041773087 hasConcept C95444343 @default.
- W2041773087 hasConceptScore W2041773087C104317684 @default.
- W2041773087 hasConceptScore W2041773087C129312508 @default.
- W2041773087 hasConceptScore W2041773087C2777166614 @default.
- W2041773087 hasConceptScore W2041773087C552990157 @default.
- W2041773087 hasConceptScore W2041773087C55493867 @default.
- W2041773087 hasConceptScore W2041773087C86803240 @default.
- W2041773087 hasConceptScore W2041773087C95444343 @default.
- W2041773087 hasLocation W20417730871 @default.
- W2041773087 hasLocation W20417730872 @default.
- W2041773087 hasOpenAccess W2041773087 @default.
- W2041773087 hasPrimaryLocation W20417730871 @default.
- W2041773087 hasRelatedWork W1481748638 @default.
- W2041773087 hasRelatedWork W1979156062 @default.
- W2041773087 hasRelatedWork W1987841739 @default.
- W2041773087 hasRelatedWork W2011329801 @default.
- W2041773087 hasRelatedWork W2020280743 @default.
- W2041773087 hasRelatedWork W2020570811 @default.
- W2041773087 hasRelatedWork W2050341469 @default.
- W2041773087 hasRelatedWork W2078334811 @default.
- W2041773087 hasRelatedWork W2091839825 @default.
- W2041773087 hasRelatedWork W2097313933 @default.
- W2041773087 hasRelatedWork W2108927848 @default.
- W2041773087 hasRelatedWork W2120598289 @default.
- W2041773087 hasRelatedWork W2135294608 @default.
- W2041773087 hasRelatedWork W2149683006 @default.
- W2041773087 hasRelatedWork W2346524739 @default.
- W2041773087 hasRelatedWork W2415310169 @default.
- W2041773087 hasRelatedWork W2897615043 @default.
- W2041773087 hasRelatedWork W2950844094 @default.
- W2041773087 hasRelatedWork W302428862 @default.
- W2041773087 hasRelatedWork W310466385 @default.
- W2041773087 isParatext "false" @default.
- W2041773087 isRetracted "false" @default.
- W2041773087 magId "2041773087" @default.
- W2041773087 workType "article" @default.