Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041799209> ?p ?o ?g. }
- W2041799209 endingPage "156" @default.
- W2041799209 startingPage "143" @default.
- W2041799209 abstract "The potassium channel Kv7.1 is expressed in the heart, where it contributes to the repolarization of the cardiac action potential. Additionally, Kv7.1 is expressed in epithelial tissues playing a role in salt and water transport. We recently demonstrated that surface-expressed Kv7.1 is internalized in response to polarization of the epithelial Madin-Darby canine kidney (MDCK) cell line and that this was mediated by activation of protein kinase C (PKC). In this study, the pathway downstream of PKC, which leads to internalization of Kv7.1 upon cell polarization, is elucidated. We show by confocal microscopy that Kv7.1 is endocytosed upon initiation of the polarization process and sent for degradation by the lysosomal pathway. The internalization could be mimicked by pharmacological activation of the AMP-activated protein kinase (AMPK) using three different AMPK activators. We demonstrate that the downstream effector of AMPK is the E3 ubiquitin ligase Nedd4-2. Additionally, we show that AMPK activation results in a downregulation of Kv7.1 currents in Xenopus oocytes through a Nedd4-2-dependent mechanism. In summary, surface-expressed Kv7.1 channels are endocytosed and sent for degradation in lysosomes by an AMPK-mediated activation of Nedd4-2 during the initial phase of the MDCK cell polarization process." @default.
- W2041799209 created "2016-06-24" @default.
- W2041799209 creator A5003737748 @default.
- W2041799209 creator A5039588653 @default.
- W2041799209 creator A5073616733 @default.
- W2041799209 creator A5076957580 @default.
- W2041799209 creator A5082541348 @default.
- W2041799209 date "2011-10-26" @default.
- W2041799209 modified "2023-10-16" @default.
- W2041799209 title "AMP-Activated Protein Kinase Downregulates Kv7.1 Cell Surface Expression" @default.
- W2041799209 cites W1944281814 @default.
- W2041799209 cites W1972659631 @default.
- W2041799209 cites W1977245174 @default.
- W2041799209 cites W1979511698 @default.
- W2041799209 cites W1980775459 @default.
- W2041799209 cites W1980932394 @default.
- W2041799209 cites W1983137097 @default.
- W2041799209 cites W1990278643 @default.
- W2041799209 cites W1991662012 @default.
- W2041799209 cites W1998470198 @default.
- W2041799209 cites W1999104552 @default.
- W2041799209 cites W2001532725 @default.
- W2041799209 cites W2002236561 @default.
- W2041799209 cites W2002422701 @default.
- W2041799209 cites W2006297035 @default.
- W2041799209 cites W2007881113 @default.
- W2041799209 cites W2010009765 @default.
- W2041799209 cites W2015633910 @default.
- W2041799209 cites W2020620553 @default.
- W2041799209 cites W2021020436 @default.
- W2041799209 cites W2022414157 @default.
- W2041799209 cites W2025582729 @default.
- W2041799209 cites W2030845841 @default.
- W2041799209 cites W2043933283 @default.
- W2041799209 cites W2048216202 @default.
- W2041799209 cites W2049713190 @default.
- W2041799209 cites W2059796847 @default.
- W2041799209 cites W2064965719 @default.
- W2041799209 cites W2065847009 @default.
- W2041799209 cites W2069270633 @default.
- W2041799209 cites W2071051861 @default.
- W2041799209 cites W2076268171 @default.
- W2041799209 cites W2077561365 @default.
- W2041799209 cites W2080028500 @default.
- W2041799209 cites W2080097627 @default.
- W2041799209 cites W2081500927 @default.
- W2041799209 cites W2082472130 @default.
- W2041799209 cites W2084240121 @default.
- W2041799209 cites W2091531959 @default.
- W2041799209 cites W2091616357 @default.
- W2041799209 cites W2102891343 @default.
- W2041799209 cites W2104605409 @default.
- W2041799209 cites W2106302419 @default.
- W2041799209 cites W2118029488 @default.
- W2041799209 cites W2123322767 @default.
- W2041799209 cites W2124097655 @default.
- W2041799209 cites W2125428479 @default.
- W2041799209 cites W2135205012 @default.
- W2041799209 cites W2136124373 @default.
- W2041799209 cites W2136901254 @default.
- W2041799209 cites W2137261534 @default.
- W2041799209 cites W2144359563 @default.
- W2041799209 cites W2147594695 @default.
- W2041799209 cites W2149307089 @default.
- W2041799209 cites W2155455801 @default.
- W2041799209 cites W2155955323 @default.
- W2041799209 cites W2158368575 @default.
- W2041799209 cites W2158809211 @default.
- W2041799209 cites W2159620999 @default.
- W2041799209 cites W2170266398 @default.
- W2041799209 cites W2170691541 @default.
- W2041799209 cites W2186962950 @default.
- W2041799209 cites W2312538957 @default.
- W2041799209 doi "https://doi.org/10.1111/j.1600-0854.2011.01295.x" @default.
- W2041799209 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21957902" @default.
- W2041799209 hasPublicationYear "2011" @default.
- W2041799209 type Work @default.
- W2041799209 sameAs 2041799209 @default.
- W2041799209 citedByCount "36" @default.
- W2041799209 countsByYear W20417992092012 @default.
- W2041799209 countsByYear W20417992092013 @default.
- W2041799209 countsByYear W20417992092014 @default.
- W2041799209 countsByYear W20417992092015 @default.
- W2041799209 countsByYear W20417992092016 @default.
- W2041799209 countsByYear W20417992092017 @default.
- W2041799209 countsByYear W20417992092018 @default.
- W2041799209 countsByYear W20417992092019 @default.
- W2041799209 countsByYear W20417992092020 @default.
- W2041799209 countsByYear W20417992092022 @default.
- W2041799209 crossrefType "journal-article" @default.
- W2041799209 hasAuthorship W2041799209A5003737748 @default.
- W2041799209 hasAuthorship W2041799209A5039588653 @default.
- W2041799209 hasAuthorship W2041799209A5073616733 @default.
- W2041799209 hasAuthorship W2041799209A5076957580 @default.
- W2041799209 hasAuthorship W2041799209A5082541348 @default.
- W2041799209 hasBestOaLocation W20417992091 @default.
- W2041799209 hasConcept C104317684 @default.
- W2041799209 hasConcept C127561419 @default.