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• W2041839118 abstract "A method is described for the preparation of viable hepatocytes from a marine fish, the plaice. Their ability to detoxify organic compounds was measured by the formation of glucuronic acid and sulphate conjugates with the model substrates 1-naphthol and phenolphthalein. 1-Naphthol was conjugated three- to four-fold faster than phenolphthalein and glucuronidation predominated with both substrates. Strong substrate inhibition of glucuronidation was observed with 200 microM 1-naphthol or phenolphthalein. No measurable sulphate conjugation was detected with phenolphthalein. Treatment of fish with 3-methylcholanthrene induced formation of both glucuronide and sulphate conjugates by two- to three-fold. Compared with rat hepatocytes, the extent of sulphation was 100-fold lower in plaice hepatocytes whereas glucuronide formation was only 10-fold lower. The observations indicate that isolated plaice hepatocytes provide a suitable system for studies of the detoxication of xenobiotic pollutants in fish liver." @default.
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• W2041839118 date "1985-11-01" @default.
• W2041839118 modified "2023-09-28" @default.
• W2041839118 title "Conjugation of organic compounds in isolated hepatocytes from a marine fish, the plaice, pleuronectes platessa" @default.
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• W2041839118 doi "https://doi.org/10.1016/0006-2952(85)90447-2" @default.
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