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- W2041861106 endingPage "78" @default.
- W2041861106 startingPage "37" @default.
- W2041861106 abstract "Nitrogen fixation within legume nodules results from a complex metabolic exchange between bacteria of the family Rhizobiaciae and the plant host. Carbon is supplied to the differentiated bacterial cells, termed bacteroids, in the form of dicarboxylic acids to fuel nitrogen fixation. In exchange, fixed nitrogen is transferred to the plant. Both the bacteroid and the plant-derived peribacteroid membrane tightly regulate the exchange of metabolites. In the bacteroid oxidation of dicarboxylic acids via the TCA cycle occurs in an oxygenlimited environment. This restricts the TCA cycle at key points, such as the 2-oxoglutarate dehydrogenase complex, and requires that inputs of carbon and reductant are balanced with outputs from the TCA cycle. This may be achieved by metabolism through accessory pathways that can remove intermediates, reductant, or ATP from the cycle. These include synthesis of the carbon polymers PHB and glycogen and bypass pathways such as the recently identified 2-oxoglutarate decarboxylase reaction in soybean bacteroids. Recent labeling data have shown that bacteroids synthesize and secrete amino acids, which has led to controversy over the role of amino acids in nodule metabolism. Here we review bacteroid carbon metabolism in detail, evaluate the labeling studies that relate to amino acid metabolism by bacteroids, and place the work in context with the genome sequences of Mesorhizobium loti and Sinorhizobium meliloti. We also consider a wider range of metabolic pathways that are probably of great importance to rhizobia in the rhizosphere, during nodule initiation, infection thread development, and bacteroid development. Referee: Dr. Robert Ludwig, Department of Molecular, Celluar, and Developmental Biology, Sinheimer Laboratories, University of California, Santa Cruz, CA 95064" @default.
- W2041861106 created "2016-06-24" @default.
- W2041861106 creator A5012154491 @default.
- W2041861106 creator A5027020196 @default.
- W2041861106 date "2003-01-01" @default.
- W2041861106 modified "2023-10-16" @default.
- W2041861106 title "Metabolism of<i>Rhizobium</i>Bacteroids" @default.
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