FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041868422> ?p ?o ?g. }

• W2041868422 endingPage "1468" @default.
• W2041868422 startingPage "1459" @default.
• W2041868422 abstract "Breast cancer has a prodigious capacity to metastasize to bone. In women with advanced breast cancer and bone metastases, bisphosphonates reduce the incidence of hypercalcaemia and skeletal morbidity. Recent clinical findings suggest that some bisphosphonates reduce the tumour burden in bone with a consequent increase in survival, raising the possibility that bisphosphonates may have a direct effect on breast cancer cells. We have investigated the in vitro effects of bisphosphonates zoledronate, pamidronate, clodronate and EB 1053 on growth, viability and induction of apoptosis in three human breast cancer cell lines (MDA-MB-231, Hs 578T and MCF-7). Cell growth was monitored by crystal violet dye assay, and cell viability was quantitated by MTS dye reduction. Induction of apoptosis was determined by identification of morphological features of apoptosis using time-lapse videomicroscopy, identifying morphological changes in nucleis using Hoechst staining, quantitation of DNA fragmentation, level of expression of bcl-2 and bax proteins and identification of the proteolytic cleavage of Poly (ADP)-ribose polymerase (PARP). All four bisphosphonates significantly reduced cell viability in all three cell lines. Zoledronate was the most potent bisphosphonate with IC50 values of 15, 20 and 3 microM respectively in MDA-MB-231, MCF-7 and Hs 578T cells. Corresponding values for pamidronate were 40, 35 and 25 microM, whereas clodronate and EB 1053 were more than two orders of magnitude less potent. An increase in the proportion of cells having morphological features characteristic of apoptosis, characteristic apoptotic changes in the nucleus, time-dependent increase in the percentage of fragmented chromosomal DNA, down-regulation in bcl-2 protein and proteolytic cleavage of PARP, all indicate that bisphosphonates have direct anti-tumour effects on human breast cancer cells." @default.
• W2041868422 created "2016-06-24" @default.
• W2041868422 creator A5025679809 @default.
• W2041868422 creator A5026098497 @default.
• W2041868422 creator A5072021346 @default.
• W2041868422 creator A5076573561 @default.
• W2041868422 creator A5082292420 @default.
• W2041868422 date "2000-04-01" @default.
• W2041868422 modified "2023-10-06" @default.
• W2041868422 title "Bisphosphonates induce apoptosis in human breast cancer cell lines" @default.
• W2041868422 cites W105289038 @default.
• W2041868422 cites W119814413 @default.
• W2041868422 cites W1906721103 @default.
• W2041868422 cites W1913723147 @default.
• W2041868422 cites W1944967894 @default.
• W2041868422 cites W1954585625 @default.
• W2041868422 cites W1984131196 @default.
• W2041868422 cites W1991208655 @default.
• W2041868422 cites W1994414479 @default.
• W2041868422 cites W1996180957 @default.
• W2041868422 cites W2007001439 @default.
• W2041868422 cites W2010298858 @default.
• W2041868422 cites W2011035915 @default.
• W2041868422 cites W2013936743 @default.
• W2041868422 cites W2022015605 @default.
• W2041868422 cites W2024306318 @default.
• W2041868422 cites W2029483636 @default.
• W2041868422 cites W2030989965 @default.
• W2041868422 cites W2031851347 @default.
• W2041868422 cites W2033294313 @default.
• W2041868422 cites W2041755164 @default.
• W2041868422 cites W2045764009 @default.
• W2041868422 cites W2058591356 @default.
• W2041868422 cites W2063176993 @default.
• W2041868422 cites W2064317544 @default.
• W2041868422 cites W2066870026 @default.
• W2041868422 cites W2072789990 @default.
• W2041868422 cites W2072858020 @default.
• W2041868422 cites W2083258979 @default.
• W2041868422 cites W2084609653 @default.
• W2041868422 cites W2092853101 @default.
• W2041868422 cites W2106142458 @default.
• W2041868422 cites W2128016549 @default.
• W2041868422 cites W2128635872 @default.
• W2041868422 cites W2133868361 @default.
• W2041868422 cites W2150045940 @default.
• W2041868422 cites W2161690532 @default.
• W2041868422 cites W2164578164 @default.
• W2041868422 cites W2274550965 @default.
• W2041868422 cites W2302517861 @default.
• W2041868422 cites W2314428698 @default.
• W2041868422 cites W2325302070 @default.
• W2041868422 cites W2336969031 @default.
• W2041868422 cites W2337075852 @default.
• W2041868422 cites W2341715540 @default.
• W2041868422 cites W2408306201 @default.
• W2041868422 cites W4235066853 @default.
• W2041868422 cites W4293247451 @default.
• W2041868422 doi "https://doi.org/10.1054/bjoc.1999.1131" @default.
• W2041868422 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2363380" @default.
• W2041868422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10780527" @default.
• W2041868422 hasPublicationYear "2000" @default.
• W2041868422 type Work @default.
• W2041868422 sameAs 2041868422 @default.
• W2041868422 citedByCount "396" @default.
• W2041868422 countsByYear W20418684222012 @default.
• W2041868422 countsByYear W20418684222013 @default.
• W2041868422 countsByYear W20418684222014 @default.
• W2041868422 countsByYear W20418684222015 @default.
• W2041868422 countsByYear W20418684222016 @default.
• W2041868422 countsByYear W20418684222017 @default.
• W2041868422 countsByYear W20418684222018 @default.
• W2041868422 countsByYear W20418684222019 @default.
• W2041868422 countsByYear W20418684222020 @default.
• W2041868422 countsByYear W20418684222021 @default.
• W2041868422 countsByYear W20418684222022 @default.
• W2041868422 countsByYear W20418684222023 @default.
• W2041868422 crossrefType "journal-article" @default.
• W2041868422 hasAuthorship W2041868422A5025679809 @default.
• W2041868422 hasAuthorship W2041868422A5026098497 @default.
• W2041868422 hasAuthorship W2041868422A5072021346 @default.
• W2041868422 hasAuthorship W2041868422A5076573561 @default.
• W2041868422 hasAuthorship W2041868422A5082292420 @default.
• W2041868422 hasBestOaLocation W20418684221 @default.
• W2041868422 hasConcept C121608353 @default.
• W2041868422 hasConcept C126322002 @default.
• W2041868422 hasConcept C142724271 @default.
• W2041868422 hasConcept C153911025 @default.
• W2041868422 hasConcept C182979987 @default.
• W2041868422 hasConcept C185592680 @default.
• W2041868422 hasConcept C18903297 @default.
• W2041868422 hasConcept C190283241 @default.
• W2041868422 hasConcept C191015642 @default.
• W2041868422 hasConcept C2776541429 @default.
• W2041868422 hasConcept C2777251235 @default.
• W2041868422 hasConcept C31573885 @default.
• W2041868422 hasConcept C43759708 @default.
• W2041868422 hasConcept C502942594 @default.

• next