Matches in SemOpenAlex for { <https://semopenalex.org/work/W2041869615> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2041869615 abstract "Bridging Integrator-1 (BIN-1), member of the BAR domain protein superfamily, has been implicated in the formation and maintenance of membrane invaginations in skeletal and cardiac muscle cells. However, it is not clear whether BIN-1 expression and t-tubule density are correlated in all circumstances. The aim of the present study was to investigate the relationship between t-tubule density and BIN-1 expression in (i) fresh atrial and ventricular myocytes (ii) ventricular myocytes cultured for 24-72 hours and (iii) ventricular myocytes from animals with monocrotaline-induced right heart failure (HF). Confocal microscopy was used to assess t-tubule density and BIN1 expression in rat myocytes using di-8-ANEPPS fluorescence or immunofluorescence respectively. In fresh ventricular myocytes, a regular t-tubule network was apparent and BIN-1 exhibited a corresponding striated pattern (n=10). In atrial myocytes t-tubules were typically absent and BIN-1 immunofluorescence was only detectable in the peripheral sarcolemma (n=20). In ventricular myocytes after 24, 48 or 72 hrs cell-culture, t-tubule density was reduced to 61 ± 1.4%, 26 ± 0.6% and 11 ± 0.5% respectively (n=10, 8 and 6, P<0.05), while mean cell area did not change significantly. Correspondingly, BIN1 expression was significantly reduced in ventricular myocytes at 48 (to 17 ± 0.14%, n=7, P<0.05) and 72 hrs (to 16 ± 0.09%; n=5, P<0.05) compared with control. In right ventricular myocytes, from rats with right HF and reduced t-tubule density, BIN1 expression decreased significantly (69 ± 1.3%; n=7, P<0.05) compared with saline-injected rats. These results suggest that where t-tubules are absent (e.g. atrial cells) or in circumstances where t-tubule density is decreased (cell culture, HF) BIN-1 expression is correspondingly reduced. This supports the hypothesis that BIN-1 has an important role in formation and maintenance of t-tubules." @default.
- W2041869615 created "2016-06-24" @default.
- W2041869615 creator A5004575280 @default.
- W2041869615 creator A5021756335 @default.
- W2041869615 creator A5041238424 @default.
- W2041869615 creator A5038435255 @default.
- W2041869615 date "2014-01-01" @default.
- W2041869615 modified "2023-09-27" @default.
- W2041869615 title "Bin-1 Expression in Normal Rat Cardiac Myocytes and in Myocytes with Reduced T-Tubule Density Due to Cell Culture or Heart Failure" @default.
- W2041869615 doi "https://doi.org/10.1016/j.bpj.2013.11.4239" @default.
- W2041869615 hasPublicationYear "2014" @default.
- W2041869615 type Work @default.
- W2041869615 sameAs 2041869615 @default.
- W2041869615 citedByCount "0" @default.
- W2041869615 crossrefType "journal-article" @default.
- W2041869615 hasAuthorship W2041869615A5004575280 @default.
- W2041869615 hasAuthorship W2041869615A5021756335 @default.
- W2041869615 hasAuthorship W2041869615A5038435255 @default.
- W2041869615 hasAuthorship W2041869615A5041238424 @default.
- W2041869615 hasBestOaLocation W20418696151 @default.
- W2041869615 hasConcept C126322002 @default.
- W2041869615 hasConcept C134018914 @default.
- W2041869615 hasConcept C159654299 @default.
- W2041869615 hasConcept C185592680 @default.
- W2041869615 hasConcept C199096232 @default.
- W2041869615 hasConcept C203014093 @default.
- W2041869615 hasConcept C207200792 @default.
- W2041869615 hasConcept C2778236600 @default.
- W2041869615 hasConcept C71924100 @default.
- W2041869615 hasConcept C86803240 @default.
- W2041869615 hasConceptScore W2041869615C126322002 @default.
- W2041869615 hasConceptScore W2041869615C134018914 @default.
- W2041869615 hasConceptScore W2041869615C159654299 @default.
- W2041869615 hasConceptScore W2041869615C185592680 @default.
- W2041869615 hasConceptScore W2041869615C199096232 @default.
- W2041869615 hasConceptScore W2041869615C203014093 @default.
- W2041869615 hasConceptScore W2041869615C207200792 @default.
- W2041869615 hasConceptScore W2041869615C2778236600 @default.
- W2041869615 hasConceptScore W2041869615C71924100 @default.
- W2041869615 hasConceptScore W2041869615C86803240 @default.
- W2041869615 hasLocation W20418696151 @default.
- W2041869615 hasOpenAccess W2041869615 @default.
- W2041869615 hasPrimaryLocation W20418696151 @default.
- W2041869615 hasRelatedWork W1980435158 @default.
- W2041869615 hasRelatedWork W1993213372 @default.
- W2041869615 hasRelatedWork W1999296029 @default.
- W2041869615 hasRelatedWork W2005654557 @default.
- W2041869615 hasRelatedWork W2024505456 @default.
- W2041869615 hasRelatedWork W2033053711 @default.
- W2041869615 hasRelatedWork W2039156083 @default.
- W2041869615 hasRelatedWork W2051103445 @default.
- W2041869615 hasRelatedWork W2065646346 @default.
- W2041869615 hasRelatedWork W2069690156 @default.
- W2041869615 hasRelatedWork W2078671382 @default.
- W2041869615 hasRelatedWork W2409838175 @default.
- W2041869615 hasRelatedWork W2415879560 @default.
- W2041869615 hasRelatedWork W2468297950 @default.
- W2041869615 hasRelatedWork W2468326935 @default.
- W2041869615 hasRelatedWork W2991422401 @default.
- W2041869615 hasRelatedWork W2993601005 @default.
- W2041869615 hasRelatedWork W3094090903 @default.
- W2041869615 hasRelatedWork W3208661184 @default.
- W2041869615 hasRelatedWork W2095502231 @default.
- W2041869615 isParatext "false" @default.
- W2041869615 isRetracted "false" @default.
- W2041869615 magId "2041869615" @default.
- W2041869615 workType "article" @default.