Matches in SemOpenAlex for { <https://semopenalex.org/work/W2042172246> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2042172246 endingPage "92" @default.
- W2042172246 startingPage "92" @default.
- W2042172246 abstract "Pharmacognosy Magazine,2011,7,26,92-96.DOI:10.4103/0973-1296.80662Published:May 2011Type:Original ArticleAuthors:Mohammad Jamshed Ahmad Siddiqui, Zhari Ismail, and Noor Hafizoh Saidan Author(s) affiliations:Mohammad Jamshed Ahmad Siddiqui, Zhari Ismail, Noor Hafizoh Saidan School of Pharmaceutical Sciences, Universiti Sains Malaysia, P. Penang 11800, Malaysia Abstract:Background: Vinca rosea (Apocynaceae) is one of the most important and high value medicinal plants known for its anticancer alkaloids. It is the iota of the isolated secondary metabolites used in chemotherapy to treat diverse cancers. Several high performance liquid chromatography (HPLC) methods have been developed to quantify the active alkaloids in the plant. However, this method may serve the purpose in quantification of V. rosea plant extracts in totality. Objective: To develop and validate the reverse phase (RP)-HPLC method for simultaneous determination of secondary metabolites, namely alkaloids from V. rosea plant extracts. Materials and Methods: The quantitative determination was conducted by RP-HPLC equipped with ultraviolet detector. Optimal separation was achieved by isocratic elution with mobile phase consisting of methanol:acetonitrile:ammonium acetate buffer (25 mM) with 0.1% triethylamine (15:45:40 v/v) on a column (Zorbax Eclipse plus C 18 , 250 mm × 4.6 mm; 5 μm). The standard markers (vindoline, vincristine, catharanthine, and vinblastine) were identified by retention time and co-injected with reference standard and quantified by external standard method at 297 nm. Results: The precision of the method was confirmed by the relative standard deviation (R.S.D.), which was lower than 2.68%. The recoveries were in the range of 98.09%-108%. The limits of detection (LOD) for each marker alkaloids were lower than 0.20 μg. Different parts of the V. rosea extracts shows different concentrations of markers, flower samples were high in vinblastine content, while methanol extract from the leaves contains all the four alkaloids in good yield, and there is no significant presence of markers in water extracts. Conclusion: HPLC method established is appropriate for the standardization and quality assurance of V. rosea plant extracts. Keywords:Catharanthine, isocratic, quality assurance, vinblastine, Vinca rosea, vincristine, vindolineView:PDF (1.08 MB) Full Text" @default.
- W2042172246 created "2016-06-24" @default.
- W2042172246 creator A5002599980 @default.
- W2042172246 creator A5050437880 @default.
- W2042172246 creator A5087453079 @default.
- W2042172246 date "2011-01-01" @default.
- W2042172246 modified "2023-09-29" @default.
- W2042172246 title "Simultaneous determination of secondary metabolites from Vinca rosea plant extractives by reverse phase high performance liquid chromatography" @default.
- W2042172246 doi "https://doi.org/10.4103/0973-1296.80662" @default.
- W2042172246 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3113361" @default.
- W2042172246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21716929" @default.
- W2042172246 hasPublicationYear "2011" @default.
- W2042172246 type Work @default.
- W2042172246 sameAs 2042172246 @default.
- W2042172246 citedByCount "19" @default.
- W2042172246 countsByYear W20421722462012 @default.
- W2042172246 countsByYear W20421722462013 @default.
- W2042172246 countsByYear W20421722462014 @default.
- W2042172246 countsByYear W20421722462015 @default.
- W2042172246 countsByYear W20421722462017 @default.
- W2042172246 countsByYear W20421722462018 @default.
- W2042172246 countsByYear W20421722462020 @default.
- W2042172246 countsByYear W20421722462021 @default.
- W2042172246 countsByYear W20421722462022 @default.
- W2042172246 countsByYear W20421722462023 @default.
- W2042172246 crossrefType "journal-article" @default.
- W2042172246 hasAuthorship W2042172246A5002599980 @default.
- W2042172246 hasAuthorship W2042172246A5050437880 @default.
- W2042172246 hasAuthorship W2042172246A5087453079 @default.
- W2042172246 hasBestOaLocation W20421722461 @default.
- W2042172246 hasConcept C116073593 @default.
- W2042172246 hasConcept C160492267 @default.
- W2042172246 hasConcept C178790620 @default.
- W2042172246 hasConcept C179998833 @default.
- W2042172246 hasConcept C185592680 @default.
- W2042172246 hasConcept C202751555 @default.
- W2042172246 hasConcept C2777096885 @default.
- W2042172246 hasConcept C2780007248 @default.
- W2042172246 hasConcept C2780782300 @default.
- W2042172246 hasConcept C2781283055 @default.
- W2042172246 hasConcept C43617362 @default.
- W2042172246 hasConcept C55493867 @default.
- W2042172246 hasConcept C556039675 @default.
- W2042172246 hasConcept C71924100 @default.
- W2042172246 hasConcept C98274493 @default.
- W2042172246 hasConceptScore W2042172246C116073593 @default.
- W2042172246 hasConceptScore W2042172246C160492267 @default.
- W2042172246 hasConceptScore W2042172246C178790620 @default.
- W2042172246 hasConceptScore W2042172246C179998833 @default.
- W2042172246 hasConceptScore W2042172246C185592680 @default.
- W2042172246 hasConceptScore W2042172246C202751555 @default.
- W2042172246 hasConceptScore W2042172246C2777096885 @default.
- W2042172246 hasConceptScore W2042172246C2780007248 @default.
- W2042172246 hasConceptScore W2042172246C2780782300 @default.
- W2042172246 hasConceptScore W2042172246C2781283055 @default.
- W2042172246 hasConceptScore W2042172246C43617362 @default.
- W2042172246 hasConceptScore W2042172246C55493867 @default.
- W2042172246 hasConceptScore W2042172246C556039675 @default.
- W2042172246 hasConceptScore W2042172246C71924100 @default.
- W2042172246 hasConceptScore W2042172246C98274493 @default.
- W2042172246 hasIssue "26" @default.
- W2042172246 hasLocation W20421722461 @default.
- W2042172246 hasLocation W20421722462 @default.
- W2042172246 hasLocation W20421722463 @default.
- W2042172246 hasLocation W20421722464 @default.
- W2042172246 hasOpenAccess W2042172246 @default.
- W2042172246 hasPrimaryLocation W20421722461 @default.
- W2042172246 hasRelatedWork W1999185620 @default.
- W2042172246 hasRelatedWork W2037599510 @default.
- W2042172246 hasRelatedWork W2061636749 @default.
- W2042172246 hasRelatedWork W2109904578 @default.
- W2042172246 hasRelatedWork W2163216423 @default.
- W2042172246 hasRelatedWork W2258252560 @default.
- W2042172246 hasRelatedWork W2353159862 @default.
- W2042172246 hasRelatedWork W2355090667 @default.
- W2042172246 hasRelatedWork W2382440586 @default.
- W2042172246 hasRelatedWork W2411519219 @default.
- W2042172246 hasVolume "7" @default.
- W2042172246 isParatext "false" @default.
- W2042172246 isRetracted "false" @default.
- W2042172246 magId "2042172246" @default.
- W2042172246 workType "article" @default.