Matches in SemOpenAlex for { <https://semopenalex.org/work/W2042185855> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2042185855 endingPage "232" @default.
- W2042185855 startingPage "227" @default.
- W2042185855 abstract "An alternative method has been developed for the isolation of both the iron molybdenum cofactor of nitrogenase (FeMoco), a small molecular weight FeMoS cluster which is the putative nitrogen- reducing site of the enzyme, and bacterioferritin, an iron storage protein similar to other ferritins, but containing heme prosthetic groups. Previously the isolation of these two species, the characterization of which is of significant current interest, has been dependent on the purification of the nitrogenase enzyme from Azotobacter vinelandii. Out new procedure eliminates the use of the anaerobic column chromatography necessary to obtain pure nitrogenase components, involving instead the heat and RNAase/ DNAase treatment of crude extracts of ruptured cells followed by sedimentation (150000 × g for 18 h) of both the 'nitrogenase complex' and bacterioferritin. The redissolved pellet from this centrifugation yields the pure crystalline bacterioferritin on addition of Mg2+. and cooling, the iron content of the protein being higher by this method than in previous reports. Likewise, denaturation by acid/base treatment of this protein mixture yields a precipitate which can be extracted with either N-methylformamide or N,N-dimethylformamide containing dithionite ion to yield solutions of FeMoco, as evidenced by UW 45 reconstitution and EPR spectral criteria. Unfortunately, preparations of FeMoco obtained by this method have a variable, but consistently low, Fe/Mo ratio and additional visible spectral features, indicating that they are significantly less pure than that those generated from purified nitrogenase. The aqueous supernatant from the denaturation also yields bacterioferritin, but with a lower iron content than that from the direct crystallization method." @default.
- W2042185855 created "2016-06-24" @default.
- W2042185855 creator A5004772621 @default.
- W2042185855 creator A5025725232 @default.
- W2042185855 creator A5045430644 @default.
- W2042185855 creator A5047569301 @default.
- W2042185855 creator A5060214259 @default.
- W2042185855 date "1988-03-01" @default.
- W2042185855 modified "2023-09-27" @default.
- W2042185855 title "A facile method of isolation for the iron molybdenum cofactor of nitrogenase and bacterial ferritin from extracts of azotobacter vinelandii" @default.
- W2042185855 cites W1007161724 @default.
- W2042185855 cites W1509852412 @default.
- W2042185855 cites W1821892404 @default.
- W2042185855 cites W1967298352 @default.
- W2042185855 cites W1968876135 @default.
- W2042185855 cites W1990147299 @default.
- W2042185855 cites W1993798891 @default.
- W2042185855 cites W1995324087 @default.
- W2042185855 cites W2002806717 @default.
- W2042185855 cites W2017174585 @default.
- W2042185855 cites W2017970644 @default.
- W2042185855 cites W2020641372 @default.
- W2042185855 cites W2027451689 @default.
- W2042185855 cites W2036442752 @default.
- W2042185855 cites W2041811746 @default.
- W2042185855 cites W2048203750 @default.
- W2042185855 cites W2050581004 @default.
- W2042185855 cites W2063289130 @default.
- W2042185855 cites W2069005428 @default.
- W2042185855 cites W2087373022 @default.
- W2042185855 cites W2123018127 @default.
- W2042185855 cites W2224930125 @default.
- W2042185855 doi "https://doi.org/10.1016/s0020-1693(00)83471-4" @default.
- W2042185855 hasPublicationYear "1988" @default.
- W2042185855 type Work @default.
- W2042185855 sameAs 2042185855 @default.
- W2042185855 citedByCount "1" @default.
- W2042185855 crossrefType "journal-article" @default.
- W2042185855 hasAuthorship W2042185855A5004772621 @default.
- W2042185855 hasAuthorship W2042185855A5025725232 @default.
- W2042185855 hasAuthorship W2042185855A5045430644 @default.
- W2042185855 hasAuthorship W2042185855A5047569301 @default.
- W2042185855 hasAuthorship W2042185855A5060214259 @default.
- W2042185855 hasConcept C13965031 @default.
- W2042185855 hasConcept C141280058 @default.
- W2042185855 hasConcept C17165728 @default.
- W2042185855 hasConcept C178790620 @default.
- W2042185855 hasConcept C179104552 @default.
- W2042185855 hasConcept C181199279 @default.
- W2042185855 hasConcept C181440489 @default.
- W2042185855 hasConcept C185592680 @default.
- W2042185855 hasConcept C197957613 @default.
- W2042185855 hasConcept C2775832776 @default.
- W2042185855 hasConcept C2776159390 @default.
- W2042185855 hasConcept C2776923230 @default.
- W2042185855 hasConcept C2777315085 @default.
- W2042185855 hasConcept C43617362 @default.
- W2042185855 hasConcept C537208039 @default.
- W2042185855 hasConcept C549387045 @default.
- W2042185855 hasConcept C55493867 @default.
- W2042185855 hasConceptScore W2042185855C13965031 @default.
- W2042185855 hasConceptScore W2042185855C141280058 @default.
- W2042185855 hasConceptScore W2042185855C17165728 @default.
- W2042185855 hasConceptScore W2042185855C178790620 @default.
- W2042185855 hasConceptScore W2042185855C179104552 @default.
- W2042185855 hasConceptScore W2042185855C181199279 @default.
- W2042185855 hasConceptScore W2042185855C181440489 @default.
- W2042185855 hasConceptScore W2042185855C185592680 @default.
- W2042185855 hasConceptScore W2042185855C197957613 @default.
- W2042185855 hasConceptScore W2042185855C2775832776 @default.
- W2042185855 hasConceptScore W2042185855C2776159390 @default.
- W2042185855 hasConceptScore W2042185855C2776923230 @default.
- W2042185855 hasConceptScore W2042185855C2777315085 @default.
- W2042185855 hasConceptScore W2042185855C43617362 @default.
- W2042185855 hasConceptScore W2042185855C537208039 @default.
- W2042185855 hasConceptScore W2042185855C549387045 @default.
- W2042185855 hasConceptScore W2042185855C55493867 @default.
- W2042185855 hasIssue "3" @default.
- W2042185855 hasLocation W20421858551 @default.
- W2042185855 hasOpenAccess W2042185855 @default.
- W2042185855 hasPrimaryLocation W20421858551 @default.
- W2042185855 hasRelatedWork W1601707852 @default.
- W2042185855 hasRelatedWork W1989508965 @default.
- W2042185855 hasRelatedWork W2008802114 @default.
- W2042185855 hasRelatedWork W2042185855 @default.
- W2042185855 hasRelatedWork W2327505358 @default.
- W2042185855 hasRelatedWork W2490664556 @default.
- W2042185855 hasRelatedWork W2951118717 @default.
- W2042185855 hasRelatedWork W4232981060 @default.
- W2042185855 hasRelatedWork W49872712 @default.
- W2042185855 hasRelatedWork W618994457 @default.
- W2042185855 hasVolume "151" @default.
- W2042185855 isParatext "false" @default.
- W2042185855 isRetracted "false" @default.
- W2042185855 magId "2042185855" @default.
- W2042185855 workType "article" @default.