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• W2042198596 abstract "In this report we present a new chemical probe, 3-HTC, that can reversibly and ratiometrically measure the thiol-disulfide equilibrium of biological systems. 3-HTC is composed of a coumarin that has a thiolate directly conjugated to its extended aromatic π system while formation of a disulfide attenuates this conjugation. The fluorescence and absorption properties of 3-HTC are therefore very sensitive to the redox state of its thiol. 3-HTC reacts reversibly with thiols and disulfides enabling its use to measure dynamic GSH/GSSH ratios in vitro as well as to monitor the reversible redox status of whole cell lysates." @default.
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• W2042198596 date "2012-01-20" @default.
• W2042198596 modified "2023-09-26" @default.
• W2042198596 title "Fluorescent Coumarin Thiols Measure Biological Redox Couples" @default.
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• W2042198596 doi "https://doi.org/10.1021/ol203105c" @default.
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