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- W2042243072 abstract "Abstract Serum samples from 15 yearling channel catfish Ictalurus punctatus convalescing after experiencing enteric septicemia of catfish were distributed into three representative serum pools, each containing equal volumes of serum from five fish. Serologic recognition of each pooled serum sample against Edwardsiella ictaluri and Escherichia coli whole cells and against secretory antigen (exoantigen) derived from E. ictaluri was measured by enzyme-linked immunosorbent assays (ELISA). Serum samples were purified by affinity chromatography with a heterologous Ra,-mutant lipopolysaccharide that was derived from rough Salmonella typhimurium TV 119 and was covalently bound to an agarose matrix. Removal of antibodies recognizing the lipopolysaccharide by cross-reactive affinity purification caused significant decreases in serologic recognition of E. ictaluri (P < 0.10) and E. coli (P < 0.01) whole cells; however, serologic recognition of the E. ictaluri-specific exoantigen was not significantly decreased. Thes..." @default.
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- W2042243072 date "1994-09-01" @default.
- W2042243072 modified "2023-09-23" @default.
- W2042243072 title "Specificity of Humoral Responses of Convalescent Channel Catfish toEdwardsiella ictaluri" @default.
- W2042243072 doi "https://doi.org/10.1577/1548-8667(1994)006<0271:sohroc>2.3.co;2" @default.
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