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• W2042540901 abstract "This paper addresses the use of bacteriophages immobilized on magnetic particles for the biorecognition of the pathogenic bacteria, followed by electrochemical magneto-genosensing of the bacteria. The P22 bacteriophage specific to Salmonella (serotypes A, B, and D1) is used as a model. The bacteria are captured and preconcentrated by the bacteriophage-modified magnetic particles through the host interaction with high specificity and efficiency. DNA amplification of the captured bacteria is then performed by double-tagging polymerase chain reaction (PCR). Further detection of the double-tagged amplicon is achieved by electrochemical magneto-genosensing. The strategy is able to detect in 4 h as low as 3 CFU mL–1 of Salmonella in Luria–Bertani (LB) media. This approach is compared with conventional culture methods and PCR-based assay, as well as with immunological screening assays for bacteria detection, highlighting the outstanding stability and cost-efficient and animal-free production of bacteriophages as biorecognition element in biosensing devices." @default.
• W2042540901 created "2016-06-24" @default.
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• W2042540901 date "2013-02-28" @default.
• W2042540901 modified "2023-09-25" @default.
• W2042540901 title "Phagomagnetic Separation and Electrochemical Magneto-Genosensing of Pathogenic Bacteria" @default.
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• W2042540901 doi "https://doi.org/10.1021/ac3024944" @default.
• W2042540901 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23406021" @default.
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