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• W2042596462 abstract "ABSTRACT Transcription from bacteriophage T4 middle promoters uses Escherichia coli RNA polymerase together with the T4 transcriptional activator MotA and the T4 coactivator AsiA. AsiA binds tightly within the C-terminal portion of the σ 70 subunit of RNA polymerase, while MotA binds to the 9-bp MotA box motif, which is centered at −30, and also interacts with σ 70 . We show here that the N-terminal half of MotA (MotA NTD ), which is thought to include the activation domain, interacts with the C-terminal region of σ 70 in an E. coli two-hybrid assay. Replacement of the C-terminal 17 residues of σ 70 with comparable σ 38 residues abolishes the interaction with MotA NTD in this assay, as does the introduction of the amino acid substitution R608C. Furthermore, in vitro transcription experiments indicate that a polymerase reconstituted with a σ 70 that lacks C-terminal amino acids 604 to 613 or 608 to 613 is defective for MotA-dependent activation. We also show that a proteolyzed fragment of MotA that contains the C-terminal half (MotA CTD ) binds DNA with a K D(app) that is similar to that of full-length MotA. Our results support a model for MotA-dependent activation in which protein-protein contact between DNA-bound MotA and the far-C-terminal region of σ 70 helps to substitute functionally for an interaction between σ 70 and a promoter −35 element." @default.
• W2042596462 created "2016-06-24" @default.
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• W2042596462 date "2002-07-15" @default.
• W2042596462 modified "2023-09-29" @default.
• W2042596462 title "The Bacteriophage T4 Transcription Activator MotA Interacts with the Far-C-Terminal Region of the σ ⁷⁰ Subunit of <i>Escherichia coli</i> RNA Polymerase" @default.
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• W2042596462 doi "https://doi.org/10.1128/jb.184.14.3957-3964.2002" @default.
• W2042596462 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/135182" @default.
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