FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2042602537> ?p ?o ?g. }

• W2042602537 endingPage "1051" @default.
• W2042602537 startingPage "1044" @default.
• W2042602537 abstract "A sulfotransferase (ST) specific to N‐21 of saxitoxin (STX) and gonyautoxin 2+3 (GTX2+3) designated as N‐ST was purified to homogeneity from the cytosolic fraction of clonal‐axenic vegetative cells of the toxic dinoflagellate Gymnodinium catenatum Graham GC21V, which causes paralytic shellfish poisoning. The enzyme transferred a sulfate group from 3′‐phosphoadenosine 5′‐phosphosulfate (PAPS) to N‐21 in the carbamoyl group of STX and GTX2+3 to produce GTX5 and C1+2, respectively. The molecular mass of the purified enzyme was determined by SDS‐PAGE to be 59 kDa. Gel filtration chromatography showed a native molecular mass of 65 kDa, indicating that the N‐ST is a monomeric enzyme. The N‐ST was specific to only N‐21 of STX and GTX2+3, and O‐22 sulfation was not observed. Moreover, the N‐ST was not active toward neo STX and GTX1+4, which differed from STX and GTX2+3, respectively, in only N‐1 hydroxylation. When various compounds previously reported to be substrates for STs in other organisms and paralytic shellfish poisoning toxins other than STX and GTX2+3 were added to the reaction mixture, N‐ST activity was not decreased. The enzyme required PAPS as the sole source of sulfate. The enzyme was optimally active at pH 6.0 and 25° C, and its activity was enhanced by Mg 2 + and Co 2 + . The K m values of the N‐ST for STX and GTX2+3 were 16.1 μM and 29.8 μM, respectively." @default.
• W2042602537 created "2016-06-24" @default.
• W2042602537 creator A5005501859 @default.
• W2042602537 creator A5019945591 @default.
• W2042602537 creator A5031980050 @default.
• W2042602537 creator A5041402895 @default.
• W2042602537 creator A5057117023 @default.
• W2042602537 creator A5072063206 @default.
• W2042602537 date "2001-12-01" @default.
• W2042602537 modified "2023-10-11" @default.
• W2042602537 title "PURIFICATION AND CHARACTERIZATION OF A SULFOTRANSFERASE SPECIFIC TO N‐21 OF SAXITOXIN AND GONYAUTOXIN 2+3 FROM THE TOXIC DINOFLAGELLATE <i>GYMNODINIUM CATENATUM</i> (DINOPHYCEAE)" @default.
• W2042602537 cites W1529162605 @default.
• W2042602537 cites W1607493519 @default.
• W2042602537 cites W1796143097 @default.
• W2042602537 cites W1872816924 @default.
• W2042602537 cites W1965254400 @default.
• W2042602537 cites W1967757121 @default.
• W2042602537 cites W1977191595 @default.
• W2042602537 cites W1983619424 @default.
• W2042602537 cites W1985626509 @default.
• W2042602537 cites W2007900090 @default.
• W2042602537 cites W2010154609 @default.
• W2042602537 cites W2013834378 @default.
• W2042602537 cites W2025658969 @default.
• W2042602537 cites W2037950717 @default.
• W2042602537 cites W2038817503 @default.
• W2042602537 cites W2049516937 @default.
• W2042602537 cites W2060280241 @default.
• W2042602537 cites W2073090426 @default.
• W2042602537 cites W2075690506 @default.
• W2042602537 cites W2086112385 @default.
• W2042602537 cites W2096555055 @default.
• W2042602537 cites W2100837269 @default.
• W2042602537 cites W2118282574 @default.
• W2042602537 cites W2122570065 @default.
• W2042602537 cites W2145003038 @default.
• W2042602537 cites W2325632497 @default.
• W2042602537 cites W2326195740 @default.
• W2042602537 cites W2332380181 @default.
• W2042602537 cites W2342959189 @default.
• W2042602537 cites W4246338188 @default.
• W2042602537 cites W4294446350 @default.
• W2042602537 cites W2417076912 @default.
• W2042602537 doi "https://doi.org/10.1046/j.1529-8817.2001.00119.x" @default.
• W2042602537 hasPublicationYear "2001" @default.
• W2042602537 type Work @default.
• W2042602537 sameAs 2042602537 @default.
• W2042602537 citedByCount "76" @default.
• W2042602537 countsByYear W20426025372012 @default.
• W2042602537 countsByYear W20426025372013 @default.
• W2042602537 countsByYear W20426025372014 @default.
• W2042602537 countsByYear W20426025372015 @default.
• W2042602537 countsByYear W20426025372016 @default.
• W2042602537 countsByYear W20426025372017 @default.
• W2042602537 countsByYear W20426025372018 @default.
• W2042602537 countsByYear W20426025372019 @default.
• W2042602537 countsByYear W20426025372020 @default.
• W2042602537 countsByYear W20426025372021 @default.
• W2042602537 countsByYear W20426025372022 @default.
• W2042602537 countsByYear W20426025372023 @default.
• W2042602537 crossrefType "journal-article" @default.
• W2042602537 hasAuthorship W2042602537A5005501859 @default.
• W2042602537 hasAuthorship W2042602537A5019945591 @default.
• W2042602537 hasAuthorship W2042602537A5031980050 @default.
• W2042602537 hasAuthorship W2042602537A5041402895 @default.
• W2042602537 hasAuthorship W2042602537A5057117023 @default.
• W2042602537 hasAuthorship W2042602537A5072063206 @default.
• W2042602537 hasConcept C142796444 @default.
• W2042602537 hasConcept C155138218 @default.
• W2042602537 hasConcept C158278297 @default.
• W2042602537 hasConcept C181199279 @default.
• W2042602537 hasConcept C18903297 @default.
• W2042602537 hasConcept C2775904581 @default.
• W2042602537 hasConcept C2777367657 @default.
• W2042602537 hasConcept C2777646408 @default.
• W2042602537 hasConcept C2778908408 @default.
• W2042602537 hasConcept C2779528183 @default.
• W2042602537 hasConcept C2779676113 @default.
• W2042602537 hasConcept C2779792404 @default.
• W2042602537 hasConcept C2780204098 @default.
• W2042602537 hasConcept C2780892065 @default.
• W2042602537 hasConcept C2781028344 @default.
• W2042602537 hasConcept C2909208804 @default.
• W2042602537 hasConcept C505870484 @default.
• W2042602537 hasConcept C55493867 @default.
• W2042602537 hasConcept C58121356 @default.
• W2042602537 hasConcept C59822182 @default.
• W2042602537 hasConcept C86803240 @default.
• W2042602537 hasConcept C89423630 @default.
• W2042602537 hasConceptScore W2042602537C142796444 @default.
• W2042602537 hasConceptScore W2042602537C155138218 @default.
• W2042602537 hasConceptScore W2042602537C158278297 @default.
• W2042602537 hasConceptScore W2042602537C181199279 @default.
• W2042602537 hasConceptScore W2042602537C18903297 @default.
• W2042602537 hasConceptScore W2042602537C2775904581 @default.
• W2042602537 hasConceptScore W2042602537C2777367657 @default.
• W2042602537 hasConceptScore W2042602537C2777646408 @default.
• W2042602537 hasConceptScore W2042602537C2778908408 @default.

• next