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• W2042622337 endingPage "152" @default.
• W2042622337 startingPage "147" @default.
• W2042622337 abstract "The agent N-methyl-N-nitrosourea (MNU) is a direct acting carcinogen and induces well-differentiated adenocarcinoma on the rat gastric mucosa. In this study, 27 histopathologically verified gastric carcinomas induced in male F344 rats were analyzed for mutations in the N-terminal phosphorylation sites (codons 1-51) of the beta-catenin gene by using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) assays. In parallel studies, the specific localization of the beta-catenin protein was also examined by immunohistochemical analysis. No mutations in the beta-catenin gene were found in any of 27 gastric carcinomas induced by MNU. Immunohistochemical analysis resulted in the beta-catenin protein to be localized in the plasma membrane but cytoplasmic and/or nuclear accumulation of beta-catenin was not identified in any of these carcinomas. These results suggest that mutations in the beta-catenin gene are less contributory to the development of rat gastric carcinomas induced by MNU. This animal model may provide a system for evaluating the mechanism of human gastric carcinogenesis that is not associated with beta-catenin gene mutations." @default.
• W2042622337 created "2016-06-24" @default.
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• W2042622337 date "2003-06-01" @default.
• W2042622337 modified "2023-09-24" @default.
• W2042622337 title "No involvement of β-catenin gene mutation in gastric carcinomas induced by N-methyl-N-nitrosourea in male F344 rats" @default.
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• W2042622337 doi "https://doi.org/10.1016/s0304-3835(03)00186-1" @default.
• W2042622337 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12767522" @default.
• W2042622337 hasPublicationYear "2003" @default.
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