FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2042672015> ?p ?o ?g. }

• W2042672015 endingPage "585" @default.
• W2042672015 startingPage "579" @default.
• W2042672015 abstract "β-adrenoceptors (βAR) play a central role in the regulation of cAMP synthesis and cardiac contractility. Nucleoside diphosphate kinase B (NDPK B) regulates cAMP signalling by complex formation with Gβγ dimers thereby activating and stabilizing heterotrimeric G(s) proteins, key transducer of βAR signals into the cell. Here, we explored the requirement of NDPK B for basal and βAR-stimulated cAMP synthesis and analysed the underlying mechanisms by comparing wild-type NDPK B (WT) and its catalytically inactive H118N mutant. Stable overexpression of both WT- and H118N-NDPK B in cardiomyocyte derived H10 cells increased the plasma membrane content of G(s) and caveolin-1 and thus enhanced the isoproterenol (ISO)-stimulated cAMP-synthesis by about 2-fold. Conversely, the loss of NDPK B in embryonic fibroblasts from NDPK A/B-depleted mice was associated with a severe reduction in membranous G(s) protein and carveolin-1 content causing a marked decrease in basal and ISO-induced cAMP formation. Re-expression of NDPK B, but not of NDPK A, was able to rescue this phenotype. Both, re-expression of WT- and H118N-NDPK B induced the re-appearance of G(s) and caveolin-1 at the plasma membrane to a similar extent. Accordingly, WT- and H118N-NDPK B similarly enhanced ISO-induced cAMP formation. In contrast, the catalytically inactive H118N-NDPK B was less potent and less effective in rescuing basal cAMP production. Identical results were obtained in neonatal rat cardiac myocytes after siRNA-induced knockdown and adenoviral re-expression of NDPK B. Our data reveal that NDPK B regulates G(s) function by two different mechanisms. The complex formation of NDPK B with G(s) is required for the stabilization of the G protein content at the plasma membrane. In addition, the NDPK B-dependent phosphotransfer reaction, which requires the catalytic activity, specifically allows a receptor-independent, basal G(s) activation." @default.
• W2042672015 created "2016-06-24" @default.
• W2042672015 creator A5032976787 @default.
• W2042672015 creator A5049262839 @default.
• W2042672015 creator A5052257826 @default.
• W2042672015 creator A5081068405 @default.
• W2042672015 creator A5091461636 @default.
• W2042672015 date "2011-03-01" @default.
• W2042672015 modified "2023-09-26" @default.
• W2042672015 title "Through scaffolding and catalytic actions nucleoside diphosphate kinase B differentially regulates basal and β-adrenoceptor-stimulated cAMP synthesis" @default.
• W2042672015 cites W1484287540 @default.
• W2042672015 cites W1544621467 @default.
• W2042672015 cites W1980836133 @default.
• W2042672015 cites W1982355483 @default.
• W2042672015 cites W1990337343 @default.
• W2042672015 cites W1995364852 @default.
• W2042672015 cites W2005614924 @default.
• W2042672015 cites W2013107306 @default.
• W2042672015 cites W2014968902 @default.
• W2042672015 cites W2018374528 @default.
• W2042672015 cites W2020589005 @default.
• W2042672015 cites W2022233074 @default.
• W2042672015 cites W2025383253 @default.
• W2042672015 cites W2030479182 @default.
• W2042672015 cites W2033472199 @default.
• W2042672015 cites W2044933108 @default.
• W2042672015 cites W2050824263 @default.
• W2042672015 cites W2075753032 @default.
• W2042672015 cites W2078748771 @default.
• W2042672015 cites W2079106772 @default.
• W2042672015 cites W2085306209 @default.
• W2042672015 cites W2108741646 @default.
• W2042672015 cites W2116048570 @default.
• W2042672015 cites W2116247281 @default.
• W2042672015 cites W2156035149 @default.
• W2042672015 cites W3375952 @default.
• W2042672015 doi "https://doi.org/10.1016/j.cellsig.2010.11.010" @default.
• W2042672015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21111809" @default.
• W2042672015 hasPublicationYear "2011" @default.
• W2042672015 type Work @default.
• W2042672015 sameAs 2042672015 @default.
• W2042672015 citedByCount "24" @default.
• W2042672015 countsByYear W20426720152013 @default.
• W2042672015 countsByYear W20426720152014 @default.
• W2042672015 countsByYear W20426720152015 @default.
• W2042672015 countsByYear W20426720152016 @default.
• W2042672015 countsByYear W20426720152017 @default.
• W2042672015 countsByYear W20426720152018 @default.
• W2042672015 countsByYear W20426720152019 @default.
• W2042672015 countsByYear W20426720152020 @default.
• W2042672015 countsByYear W20426720152022 @default.
• W2042672015 crossrefType "journal-article" @default.
• W2042672015 hasAuthorship W2042672015A5032976787 @default.
• W2042672015 hasAuthorship W2042672015A5049262839 @default.
• W2042672015 hasAuthorship W2042672015A5052257826 @default.
• W2042672015 hasAuthorship W2042672015A5081068405 @default.
• W2042672015 hasAuthorship W2042672015A5091461636 @default.
• W2042672015 hasConcept C104317684 @default.
• W2042672015 hasConcept C139407321 @default.
• W2042672015 hasConcept C153911025 @default.
• W2042672015 hasConcept C173396325 @default.
• W2042672015 hasConcept C184235292 @default.
• W2042672015 hasConcept C185592680 @default.
• W2042672015 hasConcept C55493867 @default.
• W2042672015 hasConcept C62478195 @default.
• W2042672015 hasConcept C66135807 @default.
• W2042672015 hasConcept C80631254 @default.
• W2042672015 hasConcept C86803240 @default.
• W2042672015 hasConcept C95444343 @default.
• W2042672015 hasConcept C97029542 @default.
• W2042672015 hasConceptScore W2042672015C104317684 @default.
• W2042672015 hasConceptScore W2042672015C139407321 @default.
• W2042672015 hasConceptScore W2042672015C153911025 @default.
• W2042672015 hasConceptScore W2042672015C173396325 @default.
• W2042672015 hasConceptScore W2042672015C184235292 @default.
• W2042672015 hasConceptScore W2042672015C185592680 @default.
• W2042672015 hasConceptScore W2042672015C55493867 @default.
• W2042672015 hasConceptScore W2042672015C62478195 @default.
• W2042672015 hasConceptScore W2042672015C66135807 @default.
• W2042672015 hasConceptScore W2042672015C80631254 @default.
• W2042672015 hasConceptScore W2042672015C86803240 @default.
• W2042672015 hasConceptScore W2042672015C95444343 @default.
• W2042672015 hasConceptScore W2042672015C97029542 @default.
• W2042672015 hasFunder F4320320879 @default.
• W2042672015 hasIssue "3" @default.
• W2042672015 hasLocation W20426720151 @default.
• W2042672015 hasLocation W20426720152 @default.
• W2042672015 hasOpenAccess W2042672015 @default.
• W2042672015 hasPrimaryLocation W20426720151 @default.
• W2042672015 hasRelatedWork W1978651209 @default.
• W2042672015 hasRelatedWork W2000365178 @default.
• W2042672015 hasRelatedWork W2005400238 @default.
• W2042672015 hasRelatedWork W2006332153 @default.
• W2042672015 hasRelatedWork W2096229924 @default.
• W2042672015 hasRelatedWork W2329055726 @default.
• W2042672015 hasRelatedWork W2374826051 @default.
• W2042672015 hasRelatedWork W2567613257 @default.
• W2042672015 hasRelatedWork W2735894113 @default.

• next