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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2042889847> ?p ?o ?g. }

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• W2042889847 abstract "We validated the induction of apoptosis in human prostate cancer PC3 cells by apoptosis-inducing nucleosides (AINs) released from the CD57(+)HLA-DR(bright)-natural suppressor (57.DR-NS) cell line. We analyzed the molecular signaling pathway during AINs-induced apoptosis in PC3 cells.Direct and indirect co-cultures between 57.DR-NS and PC3 cells were performed. AINs were isolated by high-performance liquid chromatography (HPLC) from 57.DR-NS cell cultures. Apoptosis in PC3 cells was analyzed by DNA fragmentation, sub-G(1) DNA content with flow cytometry, and terminal deoxynucleotide transferase-mediated dUTP nick end-labeling (TUNEL) method. The DNA strand breaks and activation of caspase-3 in PC3 cells were measured by DNA unwinding and flow cytometry assay.The 57.DR-NS cell line generated apoptosis in PC3 cells via AINs. AINs isolated from 57.DR-NS cell cultures induced apoptosis in PC3 cells. Furthermore, we found DNA strand breaks followed by activation of caspase-3 during AINs-induced apoptosis in PC3 cells.The data obtained here indicated that AINs could induce apoptosis in PC3 cells through DNA strand breaks and activation of caspase-3." @default.
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• W2042889847 date "2002-04-12" @default.
• W2042889847 modified "2023-10-03" @default.
• W2042889847 title "Human prostate cancer cell death by novel anticancer compounds, apoptosis-inducing nucleosides from CD57+HLA-DRbright natural suppressor cell line" @default.
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• W2042889847 doi "https://doi.org/10.1002/pros.10043" @default.
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