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• W2042905147 abstract "The solution speciation of metals is a critical parameter controlling the bioavailability, solution-solid phase distribution and transport of metals in soils. The natural metal-complexing ligands that exist in soil solution include inorganic anions, inorganic colloids, organic humic substances, amino acids (notably phytosiderophores and bacterial siderophores) and low-molecular mass organic acids. The latter two groups are of particular significance in the soil surrounding plant roots (the rhizosphere). A number of analytical methodologies, encompassing computational, spectroscopic, physico-chemical and separation techniques, have been applied to the measurement of the solution speciation of metals in the environment. However, perhaps with the exception of the determination of the free metal cation, the majority of these techniques rarely provide species specific information. High-performance liquid chromatography (HPLC) coupled to a sensitive detection system, such as inductively coupled plasma mass spectrometry (ICP–MS) or electrospray ionisation mass spectrometry (ESI–MS), offers the possibility of separating and detecting metal–organic acid complexes at the very low concentrations normally found in the soil environment. This review, therefore, critically examines the literature reporting the HPLC separation of metal–organic acid complexes with reference to thermodynamic equilibrium and kinetic considerations. The limitations of HPLC techniques (and the use of thermodynamic equilibrium calculations to validate analytical results) are discussed and the metal complex characteristics necessary for chromatographic separation are described." @default.
• W2042905147 created "2016-06-24" @default.
• W2042905147 creator A5090728820 @default.
• W2042905147 date "2004-12-01" @default.
• W2042905147 modified "2023-09-25" @default.
• W2042905147 title "Separation of low-molecular mass organic acid–metal complexes by high-performance liquid chromatography" @default.
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• W2042905147 doi "https://doi.org/10.1016/j.chroma.2004.10.026" @default.
• W2042905147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15628118" @default.
• W2042905147 hasPublicationYear "2004" @default.
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