FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2042950897> ?p ?o ?g. }

• W2042950897 endingPage "901" @default.
• W2042950897 startingPage "889" @default.
• W2042950897 abstract "The yeast Fus1p SH3 domain binds to peptides containing the consensus motif, R(S/T)(S/T)SL, which is a sharp contrast to most SH3 domains, which bind to PXXP-containing peptides. Here, we have demonstrated that this domain binds to R(S/T)(S/T)SL-containing peptides derived from two putative in vivo binding partners from yeast proteins, Bnr1p and Ste5p, with Kd values in the low micromolar range. The R(S/T)(S/T)SL consensus motif is necessary, but not sufficient for binding to the Fus1p SH3 domain, as residues lying N-terminal to the consensus motif also play a critical role in the binding reaction. Through mutagenesis studies and comparisons to other SH3 domains, we have discovered that the Fus1p SH3 domain utilizes a portion of the same binding surface as typical SH3 domains. However, the PXXP-binding surface, which plays the predominant role in binding for most SH3 domains, is debilitated in the WT domain by the substitution of unusual residues at three key conserved positions. By replacing these residues, we created a version of the Fus1p SH3 domain that binds to a PXXP-containing peptide with extremely high affinity (Kd = 40 nM). Based on our data and analysis, we have clearly delineated two distinct surfaces comprising the typical SH3-domain-binding interface and show that one of these surfaces is the primary mediator of almost every “non-canonical” SH3-domain-mediated interaction described in the literature. Within this framework, dramatic alterations in SH3 domain specificity can be simply explained as a modulation of the binding strengths of these two surfaces." @default.
• W2042950897 created "2016-06-24" @default.
• W2042950897 creator A5003909102 @default.
• W2042950897 creator A5052222931 @default.
• W2042950897 creator A5083613726 @default.
• W2042950897 creator A5087517360 @default.
• W2042950897 date "2008-03-01" @default.
• W2042950897 modified "2023-10-14" @default.
• W2042950897 title "Recognition of Non-canonical Peptides by the Yeast Fus1p SH3 Domain: Elucidation of a Common Mechanism for Diverse SH3 Domain Specificities" @default.
• W2042950897 cites W125596170 @default.
• W2042950897 cites W1485667500 @default.
• W2042950897 cites W1963644326 @default.
• W2042950897 cites W1965655596 @default.
• W2042950897 cites W1968204784 @default.
• W2042950897 cites W1969403795 @default.
• W2042950897 cites W1982186905 @default.
• W2042950897 cites W1982334738 @default.
• W2042950897 cites W1990516841 @default.
• W2042950897 cites W1995059751 @default.
• W2042950897 cites W1995143022 @default.
• W2042950897 cites W1998544105 @default.
• W2042950897 cites W1998700487 @default.
• W2042950897 cites W2020982738 @default.
• W2042950897 cites W2024962796 @default.
• W2042950897 cites W2028114738 @default.
• W2042950897 cites W2034699659 @default.
• W2042950897 cites W2035095688 @default.
• W2042950897 cites W2046510419 @default.
• W2042950897 cites W2056189051 @default.
• W2042950897 cites W2061636592 @default.
• W2042950897 cites W2063224782 @default.
• W2042950897 cites W2074622507 @default.
• W2042950897 cites W2074655761 @default.
• W2042950897 cites W2080426917 @default.
• W2042950897 cites W2082019380 @default.
• W2042950897 cites W2089623038 @default.
• W2042950897 cites W2095340557 @default.
• W2042950897 cites W2097497331 @default.
• W2042950897 cites W2098552577 @default.
• W2042950897 cites W2099982459 @default.
• W2042950897 cites W2104017463 @default.
• W2042950897 cites W2113754352 @default.
• W2042950897 cites W2116570139 @default.
• W2042950897 cites W2120096061 @default.
• W2042950897 cites W2125714241 @default.
• W2042950897 cites W2133115395 @default.
• W2042950897 cites W2145123833 @default.
• W2042950897 cites W2148047088 @default.
• W2042950897 cites W2152482111 @default.
• W2042950897 cites W2155549197 @default.
• W2042950897 cites W2158133029 @default.
• W2042950897 cites W2162651796 @default.
• W2042950897 doi "https://doi.org/10.1016/j.jmb.2008.01.063" @default.
• W2042950897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18280496" @default.
• W2042950897 hasPublicationYear "2008" @default.
• W2042950897 type Work @default.
• W2042950897 sameAs 2042950897 @default.
• W2042950897 citedByCount "32" @default.
• W2042950897 countsByYear W20429508972012 @default.
• W2042950897 countsByYear W20429508972013 @default.
• W2042950897 countsByYear W20429508972014 @default.
• W2042950897 countsByYear W20429508972015 @default.
• W2042950897 countsByYear W20429508972016 @default.
• W2042950897 countsByYear W20429508972017 @default.
• W2042950897 countsByYear W20429508972018 @default.
• W2042950897 countsByYear W20429508972019 @default.
• W2042950897 countsByYear W20429508972022 @default.
• W2042950897 countsByYear W20429508972023 @default.
• W2042950897 crossrefType "journal-article" @default.
• W2042950897 hasAuthorship W2042950897A5003909102 @default.
• W2042950897 hasAuthorship W2042950897A5052222931 @default.
• W2042950897 hasAuthorship W2042950897A5083613726 @default.
• W2042950897 hasAuthorship W2042950897A5087517360 @default.
• W2042950897 hasConcept C107824862 @default.
• W2042950897 hasConcept C108636557 @default.
• W2042950897 hasConcept C11960822 @default.
• W2042950897 hasConcept C12554922 @default.
• W2042950897 hasConcept C185592680 @default.
• W2042950897 hasConcept C196347352 @default.
• W2042950897 hasConcept C25166345 @default.
• W2042950897 hasConcept C2779222958 @default.
• W2042950897 hasConcept C51639874 @default.
• W2042950897 hasConcept C55493867 @default.
• W2042950897 hasConcept C70721500 @default.
• W2042950897 hasConcept C86803240 @default.
• W2042950897 hasConcept C96739231 @default.
• W2042950897 hasConceptScore W2042950897C107824862 @default.
• W2042950897 hasConceptScore W2042950897C108636557 @default.
• W2042950897 hasConceptScore W2042950897C11960822 @default.
• W2042950897 hasConceptScore W2042950897C12554922 @default.
• W2042950897 hasConceptScore W2042950897C185592680 @default.
• W2042950897 hasConceptScore W2042950897C196347352 @default.
• W2042950897 hasConceptScore W2042950897C25166345 @default.
• W2042950897 hasConceptScore W2042950897C2779222958 @default.
• W2042950897 hasConceptScore W2042950897C51639874 @default.
• W2042950897 hasConceptScore W2042950897C55493867 @default.
• W2042950897 hasConceptScore W2042950897C70721500 @default.
• W2042950897 hasConceptScore W2042950897C86803240 @default.

• next