Matches in SemOpenAlex for { <https://semopenalex.org/work/W2042976617> ?p ?o ?g. }
- W2042976617 endingPage "273" @default.
- W2042976617 startingPage "259" @default.
- W2042976617 abstract "Sperm capacitation is crucial for fertilization. However, debate continues on exactly how, where and when capacitation is elicited in the bovine female genital tract. In this study we used merocyanine-540 and the chlortetracycline (CTC) assay to test how capacitation of bull spermatozoa is affected in vitro by exposure to oviductal fluid (ODF) collected in vivo, various glycosaminoglycans (GAGs) or bicarbonate. Following different durations of exposure, spermatozoa were stained with CTC or merocyanine-540, and evaluated with epifluorescent light microscopy or flow cytometry, respectively. Incubation time did not significantly affect capacitation. Exposure (30-120 min) to ODF capacitated (p < 0.05) bull spermatozoa as measured by either merocyanine-540 or CTC. Hyaluronan was the only GAG that induced a significant increase in B-pattern spermatozoa (capacitated; p = 0.012) compared with controls. Dermatan sulphate also induced capacitation (merocyanine-540 high fluorescence; p = 0.035). Exposure to bicarbonate-enriched media also yielded an increase in merocyanine-540 high fluorescence (p < 0.0001). When bicarbonate was added to the other treatments (ODF or GAGs) an equal increase in merocyanine-540 high fluorescence was noted (p < 0.0001), compared with before addition of bicarbonate and independent of the treatment before exposure. There was no significant difference in the number of B-pattern spermatozoa when bicarbonate was added, but an significant increase in spermatozoa with an acrosome-reacted (AR)-pattern (p < 0.0001) was observed. Exposure of spermatozoa to solubilized zonae pellucidae significantly increased the AR-pattern spermatozoa (p = 0.016). In conclusion, ODF was more potent in inducing capacitation of bull spermatozoa than the individual GAGs. Our results also indicate that bicarbonate is an effector of bull sperm capacitation." @default.
- W2042976617 created "2016-06-24" @default.
- W2042976617 creator A5004971197 @default.
- W2042976617 creator A5019712192 @default.
- W2042976617 creator A5034599887 @default.
- W2042976617 creator A5061862284 @default.
- W2042976617 creator A5062584803 @default.
- W2042976617 creator A5082053162 @default.
- W2042976617 date "2006-08-01" @default.
- W2042976617 modified "2023-10-17" @default.
- W2042976617 title "<i>In vitro</i>capacitation of bull spermatozoa by oviductal fluid and its components" @default.
- W2042976617 cites W1577733629 @default.
- W2042976617 cites W1896539835 @default.
- W2042976617 cites W1966594684 @default.
- W2042976617 cites W1967006727 @default.
- W2042976617 cites W1969989312 @default.
- W2042976617 cites W1973679066 @default.
- W2042976617 cites W1977403365 @default.
- W2042976617 cites W1979271444 @default.
- W2042976617 cites W1979569894 @default.
- W2042976617 cites W1991662449 @default.
- W2042976617 cites W1992872160 @default.
- W2042976617 cites W2000460436 @default.
- W2042976617 cites W2002543176 @default.
- W2042976617 cites W2009246158 @default.
- W2042976617 cites W2014208407 @default.
- W2042976617 cites W2031759290 @default.
- W2042976617 cites W2032556510 @default.
- W2042976617 cites W2033985441 @default.
- W2042976617 cites W2039552946 @default.
- W2042976617 cites W2050619121 @default.
- W2042976617 cites W2051038137 @default.
- W2042976617 cites W2057239122 @default.
- W2042976617 cites W2060006386 @default.
- W2042976617 cites W2060651843 @default.
- W2042976617 cites W2061520890 @default.
- W2042976617 cites W2063160262 @default.
- W2042976617 cites W2064998018 @default.
- W2042976617 cites W2070922502 @default.
- W2042976617 cites W2071955606 @default.
- W2042976617 cites W2073316778 @default.
- W2042976617 cites W2077535238 @default.
- W2042976617 cites W2078177070 @default.
- W2042976617 cites W2084192092 @default.
- W2042976617 cites W2087148455 @default.
- W2042976617 cites W2095581618 @default.
- W2042976617 cites W2096125749 @default.
- W2042976617 cites W2099125631 @default.
- W2042976617 cites W2100335919 @default.
- W2042976617 cites W2100659046 @default.
- W2042976617 cites W2110744500 @default.
- W2042976617 cites W2112878750 @default.
- W2042976617 cites W2113122224 @default.
- W2042976617 cites W2116470910 @default.
- W2042976617 cites W2133265118 @default.
- W2042976617 cites W2133372541 @default.
- W2042976617 cites W2138266153 @default.
- W2042976617 cites W2145902169 @default.
- W2042976617 cites W2148069201 @default.
- W2042976617 cites W2148095920 @default.
- W2042976617 cites W2157148611 @default.
- W2042976617 cites W2168899018 @default.
- W2042976617 cites W2310803776 @default.
- W2042976617 cites W2402710513 @default.
- W2042976617 cites W3121712911 @default.
- W2042976617 cites W4230494462 @default.
- W2042976617 cites W4251144546 @default.
- W2042976617 cites W4367275815 @default.
- W2042976617 cites W4376596386 @default.
- W2042976617 doi "https://doi.org/10.1017/s0967199406003777" @default.
- W2042976617 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16822337" @default.
- W2042976617 hasPublicationYear "2006" @default.
- W2042976617 type Work @default.
- W2042976617 sameAs 2042976617 @default.
- W2042976617 citedByCount "48" @default.
- W2042976617 countsByYear W20429766172012 @default.
- W2042976617 countsByYear W20429766172013 @default.
- W2042976617 countsByYear W20429766172014 @default.
- W2042976617 countsByYear W20429766172016 @default.
- W2042976617 countsByYear W20429766172017 @default.
- W2042976617 countsByYear W20429766172018 @default.
- W2042976617 countsByYear W20429766172019 @default.
- W2042976617 countsByYear W20429766172020 @default.
- W2042976617 countsByYear W20429766172021 @default.
- W2042976617 countsByYear W20429766172022 @default.
- W2042976617 countsByYear W20429766172023 @default.
- W2042976617 crossrefType "journal-article" @default.
- W2042976617 hasAuthorship W2042976617A5004971197 @default.
- W2042976617 hasAuthorship W2042976617A5019712192 @default.
- W2042976617 hasAuthorship W2042976617A5034599887 @default.
- W2042976617 hasAuthorship W2042976617A5061862284 @default.
- W2042976617 hasAuthorship W2042976617A5062584803 @default.
- W2042976617 hasAuthorship W2042976617A5082053162 @default.
- W2042976617 hasConcept C105702510 @default.
- W2042976617 hasConcept C16685009 @default.
- W2042976617 hasConcept C178790620 @default.
- W2042976617 hasConcept C185592680 @default.
- W2042976617 hasConcept C202751555 @default.