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• W2042994824 abstract "ObjectiveTo evaluate the effect of maternal age on gene expression profile in human cumulus cells (CCs) obtained from patients underwent controlled ovarian stimulation (COS) protocol.DesignThe study includes 67 CCs isolated mechanically from mature oocytes collected from patients aged<30 years (group 1) and 79 CCs collected from patients aged ≥30 years (group 2). Both groups of CCs provided from patients underwent COS for ICSI.Materials and MethodsCCs from each mature MII oocyte were analyzed individually using whole genome U133 Plus 2.0 GeneChip Affymetrix microarrays. Significance analysis of microarray was used to analyze the array data according to age of patients.Results6118 genes were identified from 146 CC samples as present in all CC samples. Among of these genes, 41 showed a significantly different expression level between the two age group 1 and 2 respectively (P<0.05). Pathway annotation of these differentially expressed genes revealed involvement in a variety of major functional categories including metabolism, energy pathways and cell-to-cell interaction. Interestingly, a decline in transcriptional regulation like inhibitor of DNA binding 2 (x1.5, P<0.001) and proteasomal activity such as PSMA2 (x1.5, P<0.001) and PSMB9 (x1.5, P<0.001) has been observed in CCs with advanced of maternal age. In addition, the inflammatory response (CD14, x2.3) which is known to play on important role in the human pre-ovulatory were down-regulated in CCs from patients aged ≥30 years. Among the genes increased with aging, we identified and characterized a group of new CC-specific genes, calcium-dependent protein kinase (CAMK2N1) and transient receptor cation channel (TRPC4) which are significantly up-regulated (2.5-fold) in group 2 compared to group 1 (P<0.001).ConclusionThis study reveals that the expression of genes involved in transcriptional and proteasomal activities is altered in CCs with maternal age and probably explain why there is an increase in oocyte aneuploidy with age. ObjectiveTo evaluate the effect of maternal age on gene expression profile in human cumulus cells (CCs) obtained from patients underwent controlled ovarian stimulation (COS) protocol. To evaluate the effect of maternal age on gene expression profile in human cumulus cells (CCs) obtained from patients underwent controlled ovarian stimulation (COS) protocol. DesignThe study includes 67 CCs isolated mechanically from mature oocytes collected from patients aged<30 years (group 1) and 79 CCs collected from patients aged ≥30 years (group 2). Both groups of CCs provided from patients underwent COS for ICSI. The study includes 67 CCs isolated mechanically from mature oocytes collected from patients aged<30 years (group 1) and 79 CCs collected from patients aged ≥30 years (group 2). Both groups of CCs provided from patients underwent COS for ICSI. Materials and MethodsCCs from each mature MII oocyte were analyzed individually using whole genome U133 Plus 2.0 GeneChip Affymetrix microarrays. Significance analysis of microarray was used to analyze the array data according to age of patients. CCs from each mature MII oocyte were analyzed individually using whole genome U133 Plus 2.0 GeneChip Affymetrix microarrays. Significance analysis of microarray was used to analyze the array data according to age of patients. Results6118 genes were identified from 146 CC samples as present in all CC samples. Among of these genes, 41 showed a significantly different expression level between the two age group 1 and 2 respectively (P<0.05). Pathway annotation of these differentially expressed genes revealed involvement in a variety of major functional categories including metabolism, energy pathways and cell-to-cell interaction. Interestingly, a decline in transcriptional regulation like inhibitor of DNA binding 2 (x1.5, P<0.001) and proteasomal activity such as PSMA2 (x1.5, P<0.001) and PSMB9 (x1.5, P<0.001) has been observed in CCs with advanced of maternal age. In addition, the inflammatory response (CD14, x2.3) which is known to play on important role in the human pre-ovulatory were down-regulated in CCs from patients aged ≥30 years. Among the genes increased with aging, we identified and characterized a group of new CC-specific genes, calcium-dependent protein kinase (CAMK2N1) and transient receptor cation channel (TRPC4) which are significantly up-regulated (2.5-fold) in group 2 compared to group 1 (P<0.001). 6118 genes were identified from 146 CC samples as present in all CC samples. Among of these genes, 41 showed a significantly different expression level between the two age group 1 and 2 respectively (P<0.05). Pathway annotation of these differentially expressed genes revealed involvement in a variety of major functional categories including metabolism, energy pathways and cell-to-cell interaction. Interestingly, a decline in transcriptional regulation like inhibitor of DNA binding 2 (x1.5, P<0.001) and proteasomal activity such as PSMA2 (x1.5, P<0.001) and PSMB9 (x1.5, P<0.001) has been observed in CCs with advanced of maternal age. In addition, the inflammatory response (CD14, x2.3) which is known to play on important role in the human pre-ovulatory were down-regulated in CCs from patients aged ≥30 years. Among the genes increased with aging, we identified and characterized a group of new CC-specific genes, calcium-dependent protein kinase (CAMK2N1) and transient receptor cation channel (TRPC4) which are significantly up-regulated (2.5-fold) in group 2 compared to group 1 (P<0.001). ConclusionThis study reveals that the expression of genes involved in transcriptional and proteasomal activities is altered in CCs with maternal age and probably explain why there is an increase in oocyte aneuploidy with age. This study reveals that the expression of genes involved in transcriptional and proteasomal activities is altered in CCs with maternal age and probably explain why there is an increase in oocyte aneuploidy with age." @default.
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• W2042994824 title "Maternal age affects transcriptional and proteasomal activities of human cumulus cells isolated from mature MII oocyte" @default.
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