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- W2043009229 abstract "The fruit fly Drosophila is a leading model system for the study of transcriptional control by cis-regulatory elements or enhancers. Here, we present a rapid and highly efficient system for the large-scale analysis of enhancer elements, site-specifically integrated into the Drosophila genome. This system, which is scalable for either small projects or high-throughput approaches, makes use of the Gateway cloning technology and the PhiC31 site-specific integration system, which allows the insertion of constructs at predetermined genomic locations. Thus, this system allows not only a fast and easy analysis of reporter gene expression in live animals, but also the simultaneous analysis of different regulatory outputs on a cellular resolution by recombining in the same animal distinct enhancer elements fused to different fluorescent proteins." @default.
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- W2043009229 date "2010-04-22" @default.
- W2043009229 modified "2023-10-01" @default.
- W2043009229 title "Vectors for efficient and high-throughput construction of fluorescent drosophila reporters using the PhiC31 site-specific integration system" @default.
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- W2043009229 doi "https://doi.org/10.1002/dvg.20637" @default.
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