FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043018013> ?p ?o ?g. }

• W2043018013 endingPage "0" @default.
• W2043018013 startingPage "0" @default.
• W2043018013 abstract "Response surface methodology was undertaken to optimize the polyethylenimine-mediated transient transfection of suspension cultured HEK 293-F cells. A total of 15 combinations were designed according to Box-Behnken design to identify the effects of DNA concentration, polyethylenimine concentration and incubation time on transient transfection efficiency. The highest integral optic density of green fluorescent protein presenting r-protein yield was accessed using a DNA concentration of 1.75 µg/mL, a polyethylenimine concentration of 10.5 µg/mL, and an incubation time of 11.8 min. Analysis of variance demonstrated that the experimental values fit well with a quadratic model. The RSM-optimized transient transfection resulted in greater production of human tissue prokallikrein (TproK) than non-RSM-optimized conditions: protein yield was 32.0 mg/L and the maximum viable cell density reached 3.57 x 10 6 cells/mL in a 5 L stirred-tank bioreactor culture." @default.
• W2043018013 created "2016-06-24" @default.
• W2043018013 creator A5009441683 @default.
• W2043018013 creator A5059990221 @default.
• W2043018013 date "2010-09-15" @default.
• W2043018013 modified "2023-09-27" @default.
• W2043018013 title "Optimization of polyethylenimine-mediated transient transfection using response surface methodology design" @default.
• W2043018013 cites W1498641377 @default.
• W2043018013 cites W1508946059 @default.
• W2043018013 cites W1764595909 @default.
• W2043018013 cites W1788597099 @default.
• W2043018013 cites W1963910405 @default.
• W2043018013 cites W1970594627 @default.
• W2043018013 cites W2010655741 @default.
• W2043018013 cites W2012250005 @default.
• W2043018013 cites W2025636757 @default.
• W2043018013 cites W2045247083 @default.
• W2043018013 cites W2045800617 @default.
• W2043018013 cites W2053557358 @default.
• W2043018013 cites W2061933871 @default.
• W2043018013 cites W2074487444 @default.
• W2043018013 cites W2085884946 @default.
• W2043018013 cites W2130314959 @default.
• W2043018013 cites W2136476953 @default.
• W2043018013 cites W2154882802 @default.
• W2043018013 cites W2166319456 @default.
• W2043018013 cites W2187627689 @default.
• W2043018013 cites W2979202014 @default.
• W2043018013 doi "https://doi.org/10.2225/vol13-issue5-fulltext-6" @default.
• W2043018013 hasPublicationYear "2010" @default.
• W2043018013 type Work @default.
• W2043018013 sameAs 2043018013 @default.
• W2043018013 citedByCount "3" @default.
• W2043018013 countsByYear W20430180132015 @default.
• W2043018013 countsByYear W20430180132021 @default.
• W2043018013 countsByYear W20430180132022 @default.
• W2043018013 crossrefType "journal-article" @default.
• W2043018013 hasAuthorship W2043018013A5009441683 @default.
• W2043018013 hasAuthorship W2043018013A5059990221 @default.
• W2043018013 hasBestOaLocation W20430180132 @default.
• W2043018013 hasConcept C104317684 @default.
• W2043018013 hasConcept C111919701 @default.
• W2043018013 hasConcept C119599485 @default.
• W2043018013 hasConcept C127413603 @default.
• W2043018013 hasConcept C150077022 @default.
• W2043018013 hasConcept C183696295 @default.
• W2043018013 hasConcept C185592680 @default.
• W2043018013 hasConcept C186060115 @default.
• W2043018013 hasConcept C2775925850 @default.
• W2043018013 hasConcept C2780799671 @default.
• W2043018013 hasConcept C40767141 @default.
• W2043018013 hasConcept C41008148 @default.
• W2043018013 hasConcept C43617362 @default.
• W2043018013 hasConcept C54009773 @default.
• W2043018013 hasConcept C54355233 @default.
• W2043018013 hasConcept C55493867 @default.
• W2043018013 hasConcept C70721500 @default.
• W2043018013 hasConcept C81885089 @default.
• W2043018013 hasConcept C85761212 @default.
• W2043018013 hasConcept C86803240 @default.
• W2043018013 hasConcept C95444343 @default.
• W2043018013 hasConceptScore W2043018013C104317684 @default.
• W2043018013 hasConceptScore W2043018013C111919701 @default.
• W2043018013 hasConceptScore W2043018013C119599485 @default.
• W2043018013 hasConceptScore W2043018013C127413603 @default.
• W2043018013 hasConceptScore W2043018013C150077022 @default.
• W2043018013 hasConceptScore W2043018013C183696295 @default.
• W2043018013 hasConceptScore W2043018013C185592680 @default.
• W2043018013 hasConceptScore W2043018013C186060115 @default.
• W2043018013 hasConceptScore W2043018013C2775925850 @default.
• W2043018013 hasConceptScore W2043018013C2780799671 @default.
• W2043018013 hasConceptScore W2043018013C40767141 @default.
• W2043018013 hasConceptScore W2043018013C41008148 @default.
• W2043018013 hasConceptScore W2043018013C43617362 @default.
• W2043018013 hasConceptScore W2043018013C54009773 @default.
• W2043018013 hasConceptScore W2043018013C54355233 @default.
• W2043018013 hasConceptScore W2043018013C55493867 @default.
• W2043018013 hasConceptScore W2043018013C70721500 @default.
• W2043018013 hasConceptScore W2043018013C81885089 @default.
• W2043018013 hasConceptScore W2043018013C85761212 @default.
• W2043018013 hasConceptScore W2043018013C86803240 @default.
• W2043018013 hasConceptScore W2043018013C95444343 @default.
• W2043018013 hasIssue "5" @default.
• W2043018013 hasLocation W20430180131 @default.
• W2043018013 hasLocation W20430180132 @default.
• W2043018013 hasLocation W20430180133 @default.
• W2043018013 hasLocation W20430180134 @default.
• W2043018013 hasLocation W20430180135 @default.
• W2043018013 hasOpenAccess W2043018013 @default.
• W2043018013 hasPrimaryLocation W20430180131 @default.
• W2043018013 hasRelatedWork W1979578972 @default.
• W2043018013 hasRelatedWork W1999209251 @default.
• W2043018013 hasRelatedWork W2015713721 @default.
• W2043018013 hasRelatedWork W2054956516 @default.
• W2043018013 hasRelatedWork W2069945954 @default.
• W2043018013 hasRelatedWork W2091673665 @default.
• W2043018013 hasRelatedWork W2129448787 @default.
• W2043018013 hasRelatedWork W2152077699 @default.

• next