Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043040963> ?p ?o ?g. }
- W2043040963 endingPage "1769" @default.
- W2043040963 startingPage "1759" @default.
- W2043040963 abstract "ABSTRACT Research into archaea will not achieve its full potential until systems are in place to carry out genetics and biochemistry in the same species. Haloferax volcanii is widely regarded as the best-equipped organism for archaeal genetics, but the development of tools for the expression and purification of H. volcanii proteins has been neglected. We have developed a series of plasmid vectors and host strains for conditional overexpression of halophilic proteins in H. volcanii . The plasmids feature the tryptophan-inducible p. tnaA promoter and a 6×His tag for protein purification by metal affinity chromatography. Purification is facilitated by host strains, where pitA is replaced by the ortholog from Natronomonas pharaonis . The latter lacks the histidine-rich linker region found in H. volcanii PitA and does not copurify with His-tagged recombinant proteins. We also deleted the mrr restriction endonuclease gene, thereby allowing direct transformation without the need to passage DNA through an Escherichia coli dam mutant." @default.
- W2043040963 created "2016-06-24" @default.
- W2043040963 creator A5008864275 @default.
- W2043040963 creator A5034081646 @default.
- W2043040963 creator A5059245153 @default.
- W2043040963 creator A5071947773 @default.
- W2043040963 creator A5088120595 @default.
- W2043040963 date "2010-03-15" @default.
- W2043040963 modified "2023-10-01" @default.
- W2043040963 title "Improved Strains and Plasmid Vectors for Conditional Overexpression of His-Tagged Proteins in <i>Haloferax volcanii</i>" @default.
- W2043040963 cites W1482346275 @default.
- W2043040963 cites W1511926043 @default.
- W2043040963 cites W1516981487 @default.
- W2043040963 cites W1584653951 @default.
- W2043040963 cites W1970733967 @default.
- W2043040963 cites W1972044819 @default.
- W2043040963 cites W1978436270 @default.
- W2043040963 cites W1981593008 @default.
- W2043040963 cites W1987383794 @default.
- W2043040963 cites W1987827089 @default.
- W2043040963 cites W1991568949 @default.
- W2043040963 cites W1996761630 @default.
- W2043040963 cites W2000989488 @default.
- W2043040963 cites W2031753266 @default.
- W2043040963 cites W2032574084 @default.
- W2043040963 cites W2038828398 @default.
- W2043040963 cites W2046424395 @default.
- W2043040963 cites W2050433725 @default.
- W2043040963 cites W2052939618 @default.
- W2043040963 cites W2060232836 @default.
- W2043040963 cites W2070066566 @default.
- W2043040963 cites W2078371311 @default.
- W2043040963 cites W2084261089 @default.
- W2043040963 cites W2094958519 @default.
- W2043040963 cites W2112589109 @default.
- W2043040963 cites W2115806782 @default.
- W2043040963 cites W2116735948 @default.
- W2043040963 cites W2126184219 @default.
- W2043040963 cites W2139904717 @default.
- W2043040963 cites W2143527741 @default.
- W2043040963 cites W2146908577 @default.
- W2043040963 cites W2147670977 @default.
- W2043040963 cites W2151487235 @default.
- W2043040963 cites W2159520568 @default.
- W2043040963 cites W2160714701 @default.
- W2043040963 cites W2161136014 @default.
- W2043040963 cites W2161247547 @default.
- W2043040963 cites W2171101180 @default.
- W2043040963 doi "https://doi.org/10.1128/aem.02670-09" @default.
- W2043040963 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2838008" @default.
- W2043040963 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20097827" @default.
- W2043040963 hasPublicationYear "2010" @default.
- W2043040963 type Work @default.
- W2043040963 sameAs 2043040963 @default.
- W2043040963 citedByCount "170" @default.
- W2043040963 countsByYear W20430409632012 @default.
- W2043040963 countsByYear W20430409632013 @default.
- W2043040963 countsByYear W20430409632014 @default.
- W2043040963 countsByYear W20430409632015 @default.
- W2043040963 countsByYear W20430409632016 @default.
- W2043040963 countsByYear W20430409632017 @default.
- W2043040963 countsByYear W20430409632018 @default.
- W2043040963 countsByYear W20430409632019 @default.
- W2043040963 countsByYear W20430409632020 @default.
- W2043040963 countsByYear W20430409632021 @default.
- W2043040963 countsByYear W20430409632022 @default.
- W2043040963 countsByYear W20430409632023 @default.
- W2043040963 crossrefType "journal-article" @default.
- W2043040963 hasAuthorship W2043040963A5008864275 @default.
- W2043040963 hasAuthorship W2043040963A5034081646 @default.
- W2043040963 hasAuthorship W2043040963A5059245153 @default.
- W2043040963 hasAuthorship W2043040963A5071947773 @default.
- W2043040963 hasAuthorship W2043040963A5088120595 @default.
- W2043040963 hasBestOaLocation W20430409631 @default.
- W2043040963 hasConcept C104317684 @default.
- W2043040963 hasConcept C143065580 @default.
- W2043040963 hasConcept C22744801 @default.
- W2043040963 hasConcept C2776179084 @default.
- W2043040963 hasConcept C2778460671 @default.
- W2043040963 hasConcept C40767141 @default.
- W2043040963 hasConcept C515207424 @default.
- W2043040963 hasConcept C54355233 @default.
- W2043040963 hasConcept C547475151 @default.
- W2043040963 hasConcept C550995028 @default.
- W2043040963 hasConcept C55493867 @default.
- W2043040963 hasConcept C86803240 @default.
- W2043040963 hasConceptScore W2043040963C104317684 @default.
- W2043040963 hasConceptScore W2043040963C143065580 @default.
- W2043040963 hasConceptScore W2043040963C22744801 @default.
- W2043040963 hasConceptScore W2043040963C2776179084 @default.
- W2043040963 hasConceptScore W2043040963C2778460671 @default.
- W2043040963 hasConceptScore W2043040963C40767141 @default.
- W2043040963 hasConceptScore W2043040963C515207424 @default.
- W2043040963 hasConceptScore W2043040963C54355233 @default.
- W2043040963 hasConceptScore W2043040963C547475151 @default.
- W2043040963 hasConceptScore W2043040963C550995028 @default.
- W2043040963 hasConceptScore W2043040963C55493867 @default.
- W2043040963 hasConceptScore W2043040963C86803240 @default.