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- W2043046008 abstract "Abstract A rapid, fully reversible, and highly reproducible procedure for separation and quantitative detection of disease-determining phytotoxic metabolites of Alternaria alternata f.sp. lycopersici as their maleyl amide derivatives by reversed-phase high-performance liquid chromatography is described. Maleyl-derivatized toxin, prepared by reaction with maleic anhydride, with an absorbance maximum at 250 nm, was detected for 0.5–10 nmoles following chromatography on a C18 bonded-phase column using binary gradient systems. Toxin samples, maleylated at pH 9.2 and then demaleylated at pH 3.0, showed no detectable loss of biological activity after the chromatographic separation. Quantification of the procedure was based on a trinitrophenol—aspartic acid standard. The procedure is currently being used to study kinetics of toxin production in culture and qualitative detection of phytotoxin in diseased host tissue." @default.
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- W2043046008 date "1982-04-01" @default.
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- W2043046008 title "Determination of host-selective phytotoxins from Alternaria alternata f. sp. lycopersici as their maleyl derivatives by high-performance liquid chromatography" @default.
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- W2043046008 doi "https://doi.org/10.1016/s0021-9673(00)82723-5" @default.
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