Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043071185> ?p ?o ?g. }
- W2043071185 endingPage "631" @default.
- W2043071185 startingPage "625" @default.
- W2043071185 abstract "Chloroplastic glutamine synthetase (GS: EC 6·3·1·2), the octamer of the 44 kDa subunit, is rapidly degraded under photo-oxidative stress conditions in leaves, chloroplasts, and chloroplast lysates. Recent studies have suggested that chloroplastic GS might be cleaved by the hydroxyl radical under such conditions (Thoenen & Feller 1998; Australian Journal of Plant Physiology 25, 279–286; Palatnik, Carrillo & Valle 1999, Plant Physiology 121, 471–478). Herein, we present evidence which supports the above hypothesis. When the purified GS from wheat (Triticum aestivum L.) chloroplasts was exposed to the hydroxyl radical-generating system comprising H2O2–FeSO4–ascorbic acid or FeCl3–ascorbic acid, the GS subunit was degraded into four distinct fragments having apparent molecular masses of 39, 35, 32 and 28 kDa. The apparent molecular masses and isoelectric points of these fragments were identical to those of the respective fragments found in the illuminated lysates of chloroplasts. In addition, the appearance of the GS fragments was completely suppressed in the presence of the scavenger for the hydroxyl radical, n-propyl gallate, in the illuminated lysates of chloroplasts. These results strongly support the hypothesis that the primary cleavage of GS is directly driven by the hydroxyl radical, formed by Fenton reaction under photo-oxidative stress conditions in chloroplasts." @default.
- W2043071185 created "2016-06-24" @default.
- W2043071185 creator A5021242333 @default.
- W2043071185 creator A5033467196 @default.
- W2043071185 creator A5034691274 @default.
- W2043071185 creator A5047408959 @default.
- W2043071185 creator A5051917348 @default.
- W2043071185 date "2002-05-01" @default.
- W2043071185 modified "2023-09-27" @default.
- W2043071185 title "Direct evidence for non-enzymatic fragmentation of chloroplastic glutamine synthetase by a reactive oxygen species" @default.
- W2043071185 cites W1516384077 @default.
- W2043071185 cites W1529662354 @default.
- W2043071185 cites W1535086203 @default.
- W2043071185 cites W1585084333 @default.
- W2043071185 cites W1606423922 @default.
- W2043071185 cites W1608516820 @default.
- W2043071185 cites W1662761325 @default.
- W2043071185 cites W1964373090 @default.
- W2043071185 cites W1967126860 @default.
- W2043071185 cites W1992630712 @default.
- W2043071185 cites W2000559320 @default.
- W2043071185 cites W2008468314 @default.
- W2043071185 cites W2027562567 @default.
- W2043071185 cites W2045375219 @default.
- W2043071185 cites W2048839770 @default.
- W2043071185 cites W2052726502 @default.
- W2043071185 cites W2056593390 @default.
- W2043071185 cites W2062666588 @default.
- W2043071185 cites W2067332853 @default.
- W2043071185 cites W2070033664 @default.
- W2043071185 cites W2071826159 @default.
- W2043071185 cites W2081289872 @default.
- W2043071185 cites W2086009388 @default.
- W2043071185 cites W2086274333 @default.
- W2043071185 cites W2100837269 @default.
- W2043071185 cites W2103915989 @default.
- W2043071185 cites W2107919301 @default.
- W2043071185 cites W2112825024 @default.
- W2043071185 cites W2163874883 @default.
- W2043071185 cites W2895408343 @default.
- W2043071185 cites W4231064391 @default.
- W2043071185 cites W4243975953 @default.
- W2043071185 doi "https://doi.org/10.1046/j.1365-3040.2002.00851.x" @default.
- W2043071185 hasPublicationYear "2002" @default.
- W2043071185 type Work @default.
- W2043071185 sameAs 2043071185 @default.
- W2043071185 citedByCount "46" @default.
- W2043071185 countsByYear W20430711852012 @default.
- W2043071185 countsByYear W20430711852013 @default.
- W2043071185 countsByYear W20430711852014 @default.
- W2043071185 countsByYear W20430711852016 @default.
- W2043071185 countsByYear W20430711852017 @default.
- W2043071185 countsByYear W20430711852018 @default.
- W2043071185 countsByYear W20430711852019 @default.
- W2043071185 countsByYear W20430711852021 @default.
- W2043071185 countsByYear W20430711852022 @default.
- W2043071185 crossrefType "journal-article" @default.
- W2043071185 hasAuthorship W2043071185A5021242333 @default.
- W2043071185 hasAuthorship W2043071185A5033467196 @default.
- W2043071185 hasAuthorship W2043071185A5034691274 @default.
- W2043071185 hasAuthorship W2043071185A5047408959 @default.
- W2043071185 hasAuthorship W2043071185A5051917348 @default.
- W2043071185 hasConcept C104292427 @default.
- W2043071185 hasConcept C104317684 @default.
- W2043071185 hasConcept C185592680 @default.
- W2043071185 hasConcept C2776778087 @default.
- W2043071185 hasConcept C2778004101 @default.
- W2043071185 hasConcept C2779289688 @default.
- W2043071185 hasConcept C2779349466 @default.
- W2043071185 hasConcept C2986274086 @default.
- W2043071185 hasConcept C31903555 @default.
- W2043071185 hasConcept C48349386 @default.
- W2043071185 hasConcept C515207424 @default.
- W2043071185 hasConcept C55493867 @default.
- W2043071185 hasConcept C69305403 @default.
- W2043071185 hasConcept C86803240 @default.
- W2043071185 hasConceptScore W2043071185C104292427 @default.
- W2043071185 hasConceptScore W2043071185C104317684 @default.
- W2043071185 hasConceptScore W2043071185C185592680 @default.
- W2043071185 hasConceptScore W2043071185C2776778087 @default.
- W2043071185 hasConceptScore W2043071185C2778004101 @default.
- W2043071185 hasConceptScore W2043071185C2779289688 @default.
- W2043071185 hasConceptScore W2043071185C2779349466 @default.
- W2043071185 hasConceptScore W2043071185C2986274086 @default.
- W2043071185 hasConceptScore W2043071185C31903555 @default.
- W2043071185 hasConceptScore W2043071185C48349386 @default.
- W2043071185 hasConceptScore W2043071185C515207424 @default.
- W2043071185 hasConceptScore W2043071185C55493867 @default.
- W2043071185 hasConceptScore W2043071185C69305403 @default.
- W2043071185 hasConceptScore W2043071185C86803240 @default.
- W2043071185 hasIssue "5" @default.
- W2043071185 hasLocation W20430711851 @default.
- W2043071185 hasOpenAccess W2043071185 @default.
- W2043071185 hasPrimaryLocation W20430711851 @default.
- W2043071185 hasRelatedWork W1973943257 @default.
- W2043071185 hasRelatedWork W1976567836 @default.
- W2043071185 hasRelatedWork W2018548747 @default.
- W2043071185 hasRelatedWork W2027712120 @default.