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• W2043092259 abstract "Antisera eliclted by immunization of rabbits wlth 4'-aminomethyl-trioxsalen (AMT)-modified poly(A,U) complexed with methylated bovine serum albumin was characterized in competition radiolmmunoassays (RIA) and enzyme-linked immunosorbent assays (ELISA). AMT-poly(A,U) was over 10,000-fold more reactive than unmodlfied poly(A,U) or AMT alone. The antiserum cross-reacted to varying extents with AMT-modified-RNA's and -DNA's. The presence of AMT-uridine usually assured strong reactivity. The amino group of AMT contributed to antibody binding to a small degree. Binding was not significantly affected by high ionic strength, suggesting that binding does not involve ion pair formation. Murine encephalomyocarditis virus replicative intermediates, as well as cellular RNA and DNA were modified by psoralen in intact cells, suggesting that EMCV RNA and cellular RNA's in intact cells possess detectable stretches of base pairs. The antibodies described here will be useful in studying the secondary and tertiary structure of RNA's in vitro and in intact cells." @default.
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• W2043092259 date "1987-01-01" @default.
• W2043092259 modified "2023-09-26" @default.
• W2043092259 title "Antibody-nucleic acid interactions. Antibodies to psoralen-modified RNA as probes of RNA structure" @default.
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• W2043092259 doi "https://doi.org/10.1093/nar/15.21.9057" @default.
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