FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2043137075> ?p ?o ?g. }

• W2043137075 endingPage "1621" @default.
• W2043137075 startingPage "1613" @default.
• W2043137075 abstract "Abstract Mildiomycin (MIL) is a peptidyl‐nucleoside antibiotic produced by Streptoverticillum remofaciens ZJU5119 that exhibits strong inhibitory activity against powdery mildew. The entire MIL biosynthesis gene cluster was cloned and expressed in Streptomyces lividans 1326. Systematic gene disruptions narrowed down the cluster to 16 functional ORFs and identified the boundaries of the gene cluster. A putative cytosylglucuronic acid (CGA) synthase gene, milC , was disrupted in Sv. remofaciens and heterologously expressed in E. coli . An in vitro assay revealed that purified MilC could utilize either cytosine or hydroxymethylcytosine as substrate to yield CGA or hydroxymethyl‐CGA (HM‐CGA), respectively. MilG is believed to be a key enzyme in the MIL biosynthesis pathway and contains the C XXX C XX C motif characteristic of members of the radical S ‐adenosyl methionine (SAM) superfamily. Disruption of milG leads to accumulation of HM‐CGA. Labeling experiments with 13 C 6 ‐ L ‐arginine indicated that decarboxylation at C5 of the pyranoside ring was coupled with the attachment of 5‐guanidino‐2,4‐dihydroxyvalerate side chain through CC bond formation. In contrast, exogenous 13 C 6 ‐labeled 4‐hydroxy‐ L ‐arginine was not incorporated into the MIL structure. Comparative analysis of the 16 MIL ORFs with counterparts involved in the biosynthesis of the structurally similar compound blasticidin S, along with the results above, provide insight into the complete MIL biosynthetic pathway." @default.
• W2043137075 created "2016-06-24" @default.
• W2043137075 creator A5001429047 @default.
• W2043137075 creator A5027475930 @default.
• W2043137075 creator A5083738190 @default.
• W2043137075 creator A5084295430 @default.
• W2043137075 creator A5089035316 @default.
• W2043137075 date "2012-06-29" @default.
• W2043137075 modified "2023-10-17" @default.
• W2043137075 title "Analysis of the Mildiomycin Biosynthesis Gene Cluster in Streptoverticillum remofaciens ZJU5119 and Characterization of MilC, a Hydroxymethyl cytosyl-glucuronic Acid Synthase" @default.
• W2043137075 cites W1586839000 @default.
• W2043137075 cites W1940252745 @default.
• W2043137075 cites W1979707971 @default.
• W2043137075 cites W1983152536 @default.
• W2043137075 cites W1988450747 @default.
• W2043137075 cites W1990964866 @default.
• W2043137075 cites W2006540696 @default.
• W2043137075 cites W2011338816 @default.
• W2043137075 cites W2021496016 @default.
• W2043137075 cites W2022296103 @default.
• W2043137075 cites W2023950913 @default.
• W2043137075 cites W2035080843 @default.
• W2043137075 cites W2038754227 @default.
• W2043137075 cites W2042620085 @default.
• W2043137075 cites W2070309510 @default.
• W2043137075 cites W2074462548 @default.
• W2043137075 cites W2083248572 @default.
• W2043137075 cites W2093937154 @default.
• W2043137075 cites W2102136647 @default.
• W2043137075 cites W2130824852 @default.
• W2043137075 cites W2137260694 @default.
• W2043137075 cites W2142383147 @default.
• W2043137075 cites W2146420925 @default.
• W2043137075 cites W2150710339 @default.
• W2043137075 cites W2158714788 @default.
• W2043137075 cites W2164170982 @default.
• W2043137075 cites W2171555414 @default.
• W2043137075 cites W2177906561 @default.
• W2043137075 cites W2309429913 @default.
• W2043137075 doi "https://doi.org/10.1002/cbic.201200173" @default.
• W2043137075 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22753012" @default.
• W2043137075 hasPublicationYear "2012" @default.
• W2043137075 type Work @default.
• W2043137075 sameAs 2043137075 @default.
• W2043137075 citedByCount "19" @default.
• W2043137075 countsByYear W20431370752013 @default.
• W2043137075 countsByYear W20431370752014 @default.
• W2043137075 countsByYear W20431370752015 @default.
• W2043137075 countsByYear W20431370752017 @default.
• W2043137075 countsByYear W20431370752019 @default.
• W2043137075 countsByYear W20431370752020 @default.
• W2043137075 countsByYear W20431370752021 @default.
• W2043137075 countsByYear W20431370752022 @default.
• W2043137075 countsByYear W20431370752023 @default.
• W2043137075 crossrefType "journal-article" @default.
• W2043137075 hasAuthorship W2043137075A5001429047 @default.
• W2043137075 hasAuthorship W2043137075A5027475930 @default.
• W2043137075 hasAuthorship W2043137075A5083738190 @default.
• W2043137075 hasAuthorship W2043137075A5084295430 @default.
• W2043137075 hasAuthorship W2043137075A5089035316 @default.
• W2043137075 hasConcept C104317684 @default.
• W2043137075 hasConcept C181199279 @default.
• W2043137075 hasConcept C185592680 @default.
• W2043137075 hasConcept C2776414213 @default.
• W2043137075 hasConcept C2779920670 @default.
• W2043137075 hasConcept C2780201653 @default.
• W2043137075 hasConcept C36857842 @default.
• W2043137075 hasConcept C523546767 @default.
• W2043137075 hasConcept C54355233 @default.
• W2043137075 hasConcept C553450214 @default.
• W2043137075 hasConcept C55493867 @default.
• W2043137075 hasConcept C71240020 @default.
• W2043137075 hasConcept C86803240 @default.
• W2043137075 hasConceptScore W2043137075C104317684 @default.
• W2043137075 hasConceptScore W2043137075C181199279 @default.
• W2043137075 hasConceptScore W2043137075C185592680 @default.
• W2043137075 hasConceptScore W2043137075C2776414213 @default.
• W2043137075 hasConceptScore W2043137075C2779920670 @default.
• W2043137075 hasConceptScore W2043137075C2780201653 @default.
• W2043137075 hasConceptScore W2043137075C36857842 @default.
• W2043137075 hasConceptScore W2043137075C523546767 @default.
• W2043137075 hasConceptScore W2043137075C54355233 @default.
• W2043137075 hasConceptScore W2043137075C553450214 @default.
• W2043137075 hasConceptScore W2043137075C55493867 @default.
• W2043137075 hasConceptScore W2043137075C71240020 @default.
• W2043137075 hasConceptScore W2043137075C86803240 @default.
• W2043137075 hasIssue "11" @default.
• W2043137075 hasLocation W20431370751 @default.
• W2043137075 hasLocation W20431370752 @default.
• W2043137075 hasOpenAccess W2043137075 @default.
• W2043137075 hasPrimaryLocation W20431370751 @default.
• W2043137075 hasRelatedWork W1971878287 @default.
• W2043137075 hasRelatedWork W1992336064 @default.
• W2043137075 hasRelatedWork W2004879432 @default.
• W2043137075 hasRelatedWork W2056523276 @default.
• W2043137075 hasRelatedWork W2061901407 @default.
• W2043137075 hasRelatedWork W2627761893 @default.
• W2043137075 hasRelatedWork W2915106649 @default.

• next