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- W2043285855 abstract "To illustrate tandem mass spectrometry (MS/MS) as an alternative to GC/MS for the analysis of trichothecenes in solid and liquid cultures, diacetoxyscerpenol (DAS) levels were measured by GC/MS and by three MS/MS protocols. GC/MS had the lowest detection limit and better precision than the MS/MS procedures; however, GC/MS required 20-30 minutes per analysis. MS/MS was more rapid, requiring less than two minutes per analysis. DAS could be measured accurately at low nanogram levels directly from filtrates of liquid cultures. Extraction of culture filtrates simplified the identification by effectively removing an interfering matrix component which had an ion at the same m/z as the protonated DAS molecule. Use of the GC as an inlet for MS/MS was an effective way to minimize physical manipulations necessary for sample introduction. All three MS/MS procedures had similar detection limits and precisions. Occurrence levels of three trichothecenes, T-2 toxin, neosolaniol, and DAS were measured in a single analysis by sequential selection of parents for each trichothecene." @default.
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- W2043285855 date "1989-01-01" @default.
- W2043285855 modified "2023-09-25" @default.
- W2043285855 title "Analysis of trichothecene mycotoxins by mass spectrometry and tandem mass spectrometry" @default.
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- W2043285855 doi "https://doi.org/10.1016/s0040-4020(01)83430-x" @default.
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